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Design And Implementation Of Electrical Control And Software System For Progesterone Detector

Posted on:2021-01-13Degree:MasterType:Thesis
Country:ChinaCandidate:L YangFull Text:PDF
GTID:2404330611996646Subject:Biomedical engineering
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With the promulgation of the second child policy in 2010,more and more families have joined the fertility plan,and woman's reproductive health issues have received more attention than ever.Especially for older women,the fertility problems they face are particularly prominent and timely.Understanding the changes in hormones in the body and adopting coping strategies in a timely manner is the first step to ensure their health.Fluorescence immunochromatography is an immune detection technology that has emerged in recent years.It has played an important role in food and medical safety detection due to its advantages such as high sensitivity and rapid quantitative detection.This thesis first analyzes the advantages and disadvantages of different current hormone detection products,after studying the principles of fluorescence immunochromatography,a portable progesterone monitoring device based on fluorescence immunochromatography of nano is proposed and designed to achieve immediate detection of Luteinizing Hormone(LH),Follicle-stimulating Hormone(FSH),and urine Progesterone(P).The tester uses a bipolar stepper motor of a screw rod to push the test strip in the slot of the one-dimensional motion platform forward.The epitaxial optical system is used to irradiate a light source with a wavelength of 365 nm to a moving test strip,so that it excites fluorescence at a wavelength of 615 nm.In terms of electrical control system design,the detector uses STM32F407ZGT6 as the main control chip,and a controllable laser drive circuit is designed.A high-sensitivity photoelectric sensor is used to convert the collected fluorescent signals of different intensity into corresponding current values.Then,the output current value is preprocessed by the I/V conversion circuit and the amplification filter circuit,and then sent to the microcontroller for AD acquisition.The instrument also expands rich peripheral resources,including integrated micro-embedded scanner,4.3-inch touch screen,micro printer,Bluetooth serial port,EEPROM,SD card.In terms of software system design,the AD acquisition initialization settings are first introduced.Designed to directly send the sampled value to the memory through DMA transfer to reduce the frequency of MCU use.At the same time,the hardware is equipped with a small real-time operating system UCOSIII,and the program is divided into multiple sub-tasks using the multi-tasking mechanism of UCOSIII,and combined with STemVin to write a concise human-computer interaction interface.Finally,the software driver writing for each part of the hardware is completed.Finally,the original waveform signal after digital-to-analog conversion is digitally filtered,and the wavelet transform is used to denoise the ratios at the C and T lines by the area method.The St/Sc value is linearly fitted to the experimental sample concentration to obtain The concentration detection equations of different hormone items are stored in the detector for later use.The LH,FSH,and P samples were configured to test the repeatability and accuracy of the instrument.The results show that the detector has high accuracy,good real-time performance,and a coefficient of variation <2%,which can better meet the requirements of the application field.
Keywords/Search Tags:progesterone, fluorescent immunochromatography, quantitative detection, STM32F407ZGT6, UCOSIII
PDF Full Text Request
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