Expression Of ASPM And H2AFZ In Hepatocellular Carcinoma And Their Relationship With Prognosis Of Liver Transplantation For Hepatocellular Carcinoma

PART? Bioinformatics Analysis of Liver Cancer Related GenesObjective: To analyze the transcriptional genome chip data set of hepatocellular carcinoma(HCC)by bioinformatics technology to find the key genes closely related to the occurrence and development of HCC,to explore the mechanism of HCC occurrence and development,to find biomarkers that can predict the survival of HCC patients,and to provide a new direction for the diagnosis and treatment of HCC.Methods: The published liver cancer gene chip data sets(GSE 4520-3921,GSE14520-571,GSE39791,GSE57957,GSE76427)downloaded from GEO database were used for data mining analysis on the occurrence and development of liver cancer.GE02 R program was used to screen differentially expressed genes.DAVID software is used to perform GO function analysis and KEGG signal pathway enrichment analysis on differentially expressed genes.Finally,STRING software is used to perform protein-protein interaction network analysis on proteins encoded by differentially expressed genes to find key genes in the network,and TCGA database is combined to test the effect of the above key genes on survival time of liver cancer patients.Results: 151 differentially expressed genes were screened out,including 22 differentially expressed genes up-regulated and 129 differentially expressed genes down-regulated.The GO functional enrichment analysis showed that both up-regulated and down-regulated differential genes were enriched in extracellular exosomes.The enrichment analysis of KEGG signal pathway shows that up-regulated genes are mainly enriched in cell cycle,PI3K-AKT signal pathway,ECM receptor interaction,etc.Down-regulated differential genes are mainly distributed in metabolic pathways.Through the survival analysis of the screening surface of the key genes in the protein-protein interaction network at the central node combined with TCGA database,it is finally found that the 7 genes ASPM,PRC1,TOP2 A,CDC20,H2 AFZ,PTTG1,NUSAP1 are significantly related to the overall survival rate of liver cancer.Conclusion: Cell cycle,PI3K-AKT signaling pathway and ECM receptor interaction may be closely related to the occurrence and development of liver cancer.ASPM,PRC1,TOP2 A,CDC20,H2 AFZ,PTTG1,NUSAP1 are related to the poor prognosis of liver cancer patients and can be used as biomarkers for predicting the prognosis of liver cancer patients.PART ? Expression of ASPM and H2 AFZ and Prognosis of Liver Transplantation for Hepatocellular CarcinomaObjective: Liver cancer is one of the most common malignant tumors in the world.The incidence and mortality of liver cancer are high in China.At present,liver transplantation is one of the effective methods to treat liver cancer,but the recurrence rate of liver cancer after liver transplantation is still high.Some studies have shown that ASPM and H2 AFZ are highly expressed in various tumor tissues and play an important role in the occurrence and development of tumors.However,there are few related studies on ASPM and H2 AFZ in liver cancer tissues.Therefore,this study uses immunohistochemical method to analyze the expression of ASPM and H2 AFZ in liver cancer tissues,and explores the relationship between the expression levels of ASPM and H2 AFZ and the clinicopathological characteristics of liver cancer and the prognosis of patients after liver transplantation for liver cancer.Methods: Immunohistochemical method was used to detect the expression of ASPM and H2 AFZ in 72 liver cancer tissue specimens and 36 adjacent tissue specimens of liver cancer liver transplantation patients who met Hangzhou standard.Combined with clinical pathological data,the correlation between the expression level of ASPM and H2 AFZ in liver cancer tissue and clinical pathological characteristics,prognosis after liver transplantation for liver cancer was statistically analyzed.Results:Immunohistochemical results showed that the high expression rates of ASPM in liver cancer tissues and adjacent tissues were 58.3% and 25.0%,respectively,with statistically significant difference(p < 0.05).the high expression rate of ASPM in liver cancer tissues was significantly higher than that in adjacent tissues.The high expression rates of H2 AFZ in liver cancer tissues and adjacent tissues were 48.6% and 13.9%,respectively,with statistically significant difference(p < 0.05).the high expression rate of H2 AFZ in liver cancer tissues was significantly higher than that in adjacent tissues.The expression level of ASPM has no correlation with the patient's sex,age,smoking history,drinking history,hepatitis history,preoperative AFP level,tumor size,tumor number and vascular tumor thrombus(p > 0.05),while the high expression rates of ASPM in the postoperative pathological differentiation type I-II group and III-IV group are 51.0% and76.2%(P=0.049),respectively,with statistically significant differences.the higher the pathological differentiation of liver cancer,the higher the expression level of ASPM in liver cancer;However,the expression level of H2 AFZ in liver cancer tissue has no correlation with the patient's sex,age,smoking history,drinking history,hepatitis history,preoperative AFP level,tumor size,tumor number,vascular tumor thrombus,and pathological differentiation type(P > 0.05).The median follow-up time of 72 liver cancer patients in this study was 29 months,of which 20 patients relapsed and 8 died after liver transplantation.Kaplan-Meier survival analysis showed that the 1-year,3-year and 5-year disease-free survival rates of ASPM high expression group and low expression group in liver cancer tissues were 78.6%,55.5%,55.5% and 86.3%,86.3%,86.3%,respectively.The difference was statistically significant(P=0.036).The disease-free survival rate of high expression group was lower.However,the 1-,3-,and 5-year overall survival rates were 97.6%,80.6%,80.6%,and 93.3%,89.7%,and 89.7%,respectively,with no significant difference(P > 0.05).In liver cancer tissues,the 1-year,3-year and 5-year disease-free survival rates of H2 AFZ high expression group and low expression group were 74.3%,57.1%,57.1% and 88.9%,79.5% and 79.5%,respectively,with statistically significant difference(P=0.037),while the disease-free survival rate of high expression group was lower.However,the 1-,3-,and 5-year overall survival rates were 94.3%,78.1%,78.1%,and 97.3%,90.8%,and 90.8%,respectively,with no significant difference(P >0.05).Conclusion: The expression of ASPM and H2 AFZ in liver cancer tissues is significantly higher than that in adjacent tissues.The expression level of ASPM in liver cancer tissue is related to the pathological differentiation type of liver cancer.High expression of ASPM and H2 AFZ in liver cancer tissue can reduce the tumor-free survival rate of liver cancer liver transplant patients,and can be used as a biomolecular marker for predicting the adverse prognosis of liver cancer liver transplant patients.