Changes Of Serum MOTS-c In Patients With Coronary Heart Disease And Its Relationship With Degree Of Coronary Artery Stenosis

Objective: MOTS-c is the Mitochondrial derived peptides discovered in 2015.It is a bioactive short peptide encoded by mitochondrial DNA with protective cells,insulin sensitization,anti-inflammatory and other effects.Preliminary study shows MOTS-c play a role in the process of diabetes,obesity,inflammation,osteoporosis,aging and other diseases.A recent study found that the level of MOTS-c in serum samples collected from patients with coronary artery endothelial dysfunction was positively correlated with endothelial function.Therefore,MOTS-c is likely to become a new target for the diagnosis and treatment of coronary heart disease.The purpose of our experiment is to explore the changes of MOTS-c level in serum of patients with coronary heart disease,analyze the relationship between MOTS-c and the severity of coronary lesions,and explore its clinical significance.Method: A total of 500 patients who underwent coronary angiography in the department of cardiology of our hospital from January 2018 to December 2018 were selected.A total of 60 patients with no significant differences in age,gender,weight,height,body mass index,history of hypertension,history of diabetes,history of smoking and history of drinking were selected from the 500 patients.Among them,20 patients with stable angina pectoris(SAP group),20 patients with acute myocardial infarction(AM I group)and 20 patients with normal coronary angiography(NC group).The general data were collected.The venous blood samples was sent to the hospital laboratory.The left ventricular ejection fraction was measured by color Doppler echocardiography.Coronary angiography was performed by two interventional cardiologists in our hospital who were not involved in the trial design,and the results were objectively recorded for later analysis.The severity of coronary stenosis was scored using the Gensini scoring system.The level of serum MOTS-c was measured by enzyme-linked immunosorbent assay.Spss22.0 statistical software was used for analysis.Results were presented as number(percent,%)for categorical variables and mean ± standard deviation for continuous variables.The comparisons between groups were conducted by the 2 test or 1-way analysis of variance(ANOVA)with LSD's post hoc test set.Pearson correlation coefficient was used for correlation analysis.When p<0.05,the difference was statistically significant.Result:(1)Comparison of general data among groups,There were significant differences in leukocyte count,LVEF,G score and BNP among the three groups(P<0.05).The others were no significant difference among the three groups(P>0.05).(2)Comparison of serum MOTS-c levels among groups,The serum MOTS-c level in SAP group an d AMI group was significantly lower than that in NC group,and the serum MOTS-c level in AMI group was significantly lower than that in SAP group(P<0.05).(3)There was a negative correlation between serum MOTS-c level and Gensini score in patients with CHD(r=-0.354,P=0.025).There was a positive correlation between serum MOTS-c level and LDL-C level(r=0.411,P=0.008)and between serum MOTS-c level and TC level(r=0.373,P=0.018)in CHD patients.There was no significant correlation between serum MOTS-c level with age,BMI,systolic blood pressure,diastolic blood pressure,urea nitrogen,creatinine,FBG,TG,HDL,WBC,LVEF,BNP(P>0.05).Conclusion: Our study for the first time revealed that the serum MOTS-c level in patients with coronary heart disease decreased,and was negatively correlated with the severity of coronary lesions,suggesting that the low level of MOTS-c may be related to the occurrence and development of CHD.This study provides evidence to further explore the relationship between mitochondrial damage and atherosclerosis.MOTS-c may become a new target for the treatment of coronary heart disease.