Study On The Impact Of Nattokinase On Sclerotin Of Rat With Osteoporosis Caused By Alcohol

ObjectiveThe purpose of this paper is to study the impact of nattokinase on sclerotin of rat with osteoporosis caused by alcohol.MethodThe method was to select 60 2-month-old male rats and,based on their weight,randomly divide them into four groups: normal group,alcohol model group,nattokinase control group,and nattokinase treatment group.The normal group were given normal saline once a day.The alcohol model group were given 8ml of 50%(V/V)alcohol every day for two weeks,and 12ml/(kg·bw·d)for the next six weeks.The nattokinase control group were given 530.39(fu/g)of nattokinase capsule solution every day.The nattokinase treatment group were given 530.39(fu/g)of nattokinase capsule solution and the same amount of alcohol as the alcohol model group for eight weeks.Use ICP-AES to detect the level of blood Ca and P,ELISA to detect the level of BGP and BMP-2,H&E staining to observe the pathologic change of the rat femurs,DEXA to detect BMD of the rat femurs.The following results were obtained: Result1.The alcohol model group showed a significantly lower level of BMD than the normal group(P <0.05),and BMD level of the nattokinase treatment group was obviously higher than the alcohol model group(P <0.05);2.The H&E staining results showed that bone trabecula of the normal group was dense,regular and thick,with an even thickness;bone trabecula of the alcohol model group was discrete,irregular,with an uneven thickness and even fractures;bone trabecula of the nattokinase treatment group was dense,regular and thick,with an even thickness,no fracture was found;3.Blood serum test results showed that the level of blood BGP,BMP-2,Ca and P of the alcohol model group were significantly lower than the normal group(P <0.05);the nattokinase control group and normal group showed similar blood serum rest results;the level of blood BGP,Ca and P of the nattokinase treatment group were significantly higher than the alcohol model group((P <0.05).4.The results of serum endotoxin indicators showed that: Compared with the control group,there was no significant change in nattokinase control group,the level of serum endotoxin in model group increased significantly(P<0.05).Compared with model group,the level of serum endotoxin decreased significantly in Nattokinase intervention group(P<0.05).ConclusionThe study led to the following conclusion: rat with AOP suffers from significantly decreased bone formation and bone mineral loss;Nattokinase can help increase bone formation of rat with AOP and restrain bone mineral loss and has an obvious protective effect on osteoporosis treatment inrat.