Effect Of Exenatide On Intrauterine Adhesion Mice And Its Mechanism Research

Objective: The purpose of this experiment is to explore the effect of Exenatide on intrauterine adhesions(IUAs)and its mechanism,so as to provide new ideas for the clinical treatment of IUAs.Methods: In this experiment,IUAs animal model was established by double stimulation of mechanical curettage and inflammation.After 1 week of modeling,the treatment group was injected subcutaneously with three doses of Exenatide for two weeks,and the model group was injected with sterile ultrapure water for two weeks;the sham operation group was treated the same as the normal group except for the observation of abdominal wound changes.After the treatment,the mice were sacrificed by cervical dysfunction.Obtained mouse uterine tissue will be used for subsequent experimental detection,using HE and Masson staining for histomorphological and pathological analysis,qRt-PCR detection of TGF-?1,?-SMA and MMP-9 gene expression in uterine tissue,Immunohistochemical analysis of TGF-?1,?-SMA and MMP-9 protein expression to verify whether Exenatide has a therapeutic effect on IUAs in mice.Results: In the high-dose exenatide treatment group,the endometrial glands of the mice increased significantly,and the deposition area of collagen fibers in the endometrial tissue was significantly reduced.In addition,we observed that the expression of TGF-?1 and ?-SMA in the endometrial tissue of IUAs mice in this group was significantly reduced,and the expression of MMP-9 was significantly increased.Conclusion: Exenatide may reduce the deposition of collagen fibers in the uterus of IUAs mice and promote the proliferation of endometrial glands in mice by regulating the expression of TGF-?1,?-SMA,and MMP-9,thereby improving the degree of fibrosis in the mouse uterus.