Study On Cultivation,Identification And Cerebrovascular Imaging Of Transgenic Tumor-bearing ND2:SmoA1 Mice

Malignant intracranial tumor is one of the malignant tumors with the highest lethality rate,among which medulloblastoma is the most common malignant tumor of nervous system in children.For cancer research,the creation of reliable dis ease models on mice plays an important role in the development and improvement of disease diagnosis and treatment.At present,for malignant intracranial t umors,surgical intervention is still the key method for preventing and treating cancer symptoms.Studies have shown that extensive and precise surgical resection can greatly extend the survival of patients with brain tumors,especially those with high-grade malignant gliomas.In recent years,the use of tumor fluorescent markers to achieve tumor diagnosis and real-time imaging-guided surgical resection has become an important technology to improve tumor removal accuracy and patient survival.Semiconductor semiconducting nanoparticles(SPN)have attracted considerable attention because of their many outstanding advantages as fluorescent probes,and have great potential in this field.The main content of this article is the breeding of the Jackson Laboratory medulloblastoma model,ND2:SmoA1 transgenic mice and the identification of genes and phenotypes,which proves the model's ability to produce brain tumors.Afterwards,an intravenous two-region infrared imaging experiment based on synthetic semiconductor conjugated polymer nanomaterials was performed on the brain blood vessels of t umor-bearing mice,which proved the brain blood vessel imaging ability of the material and the primary intracranial malignancy The application potential of tumor models in developing real-time imaging systems for brain tumors.In this paper,the semiconductor conjugated polymer nanomaterials were synthesized by nanoprecipitation method[2],and their absorption and emission and biocompatibility characterization experiments proved that they have high quantum efficiency and excellent infrared two-region imaging capability.When breeding mice of this strain,homozygous ND2:SmoA1 male mice were mated with C57 wild-type female mice,and homozygous ND2:SmoA1 mice were screened by progeny self-crossing and quantitative PCR gene identification technology.Compared with the method of directly mating and breeding offspring using homozygous ND2:SmoA1 mice,it greatly increases the expansion rate of mice while avoiding the risk of ND2: SmoA1 homozygous female mice due to brain tumors during pregnancy and feeding offspring.On this basis,this paper completed the infrared two-region fluorescence imaging experiment on the brain of early and late tumor-bearing mice.Comparison with the data of normal mice in the control group can cl early distinguish whether the mice are tumor-bearing and verified by pathological section.In the future,with this model and advanced semiconductor nano-imaging materials,it is expected to further develop an accurate and reliable intracranial tumor real-time imaging system.