Biological Role Of LncRNA AK093407 In Colorectal Cancer

The incidence of colorectal cancer is increasing year by year,and the detection of related molecular targets is of great significance for its diagnosis and treatment.long noncoding RNA(long noncoding RNA s,lnc RNAs)can play a regulatory role before and after genome transcription,and epigenetic regulation is involved in the process of tumorigenesis and development.At present,a large number of lnc RNAs have been screened and confirmed to be closely related to tumorigenesis and development,showing great potential in tumor diagnosis and targeted therapy.Among colorectal cancer,scholars have studied the screening and biological function of related lnc RNAs,but it is still in its infancy,and its role and mechanism need to be systematically and deeply studied.we examined the expression levels of lnc RNA AK093407 in colorectal cancer tissues and analyzed their expression differences in cancer tissues and paracancerous tissues;constructed AK093407 low-expression HCT-15 ? HCT-116 cell model by silencing AK093407(abbreviated as AK093407)to observe the effects of low expression on cell proliferation,cycle and apoptosis;studied the effects of AK093407 on the expression of protein factors associated with cycle and apoptosis,and preliminarilyexplored the possible mechanism of AK093407 in colorectal cancer.The present study examined the biological role of AK093407 in human colorectal cancer from both tissue and cell levels.It aims to provide a theoretical basis for the study of the role of AK093407 in colorectal cancer and to provide new molecular targets for the diagnosis and treatment of colorectal cancer.Objective: To observe the biological role of AK093407 in colorectal cancer and initially explore the relevant mechanisms of AK093407 in colorectal cancer cells.Methods: q RT-PCR was used to detect the expression levels of AK093407 in rectal cancer tissues and colorectal cancer cells HCT-15,HCT-116;by transfection of small interfering RNA(si RNA)silencing AK093407 in colorectal cancer cells HCT-15,HCT-116;MTT experiment was used to detect cell proliferation;annexin V-FITC/PI staining method was used to detect apoptosis;PI staining method was used to detect cell cycle;protein imprint test was used to detect the expression of apoptotic related protein Caspase3,Caspase8,Caspase9,Bax,Bcl-2,cell cycle-related protein Cylin-A1,Cylin-B1,Cylin-D1,Cylin-E1,P21,P27.Results: The expression of AK093407 in rectal cancer tissues was higher than that in paracancerous tissues;the expression of AK093407 in colorectal cancer cells hct-15,hct-116 was higher thanthat in normal colorectal cancer cells nm460;after silencing AK093407,the proliferation activity of hct-15,hct-116 cells was decreased and apoptosis was enhanced.The cell cycle was blocked at G1/S stage;apoptosis-related proteins Caspase3,Caspase8,Caspase9,Bax expression was increased,bcl-2 expression was decreased,and the expression of Cylin-A1,Cylin-B1,P21,P27 expression was increased and Cylin-D1,Cylin-E1 expression was decreased.Conclusion:AK093407 was highly expressed in colorectal cancer tissues and hct-15,hct-116 cells.silencing AK093407 could inhibit cell proliferation,induce apoptosis and cell cycle arrest.The inhibition of AK093407 is accompanied by the activation of endogenous and exogenous activation pathways of apoptosis,and the inhibition of JAK-STAT3 signaling pathway may also be possible by inhibiting STAT3 phosphorylation by altering the cycle-related factors...