| Objective: In this study,we investigated the expressions and their correlations of Linc01021 and mi R-576/mi R425 in paired of colorectal cancer and tumor-adjacent normal control tissues(n = 157).Furthermore,we analyzed the correlation of Linc01021,mi R-576 and mi R425 with clinicopathological parameters,prognostic survival times of these included colorectal cancer patients receiving oxaliplatin-based chemotherapy.Methods: The in-situ hybridization histochemistry(ISH)method was performed to detect the expression level and intracellular localization of Linc01021,mi R-576 and mi R-425 in this included 132 colorectal cancer patients who receiving oxaliplatin-based chemotherapy.The T test of two independent samples,Pearson ?2 test,and logistic regression were used to compares the expression differences of the three indicators between these two groups.The data are presented as mean ± standard deviation(s.d.)or median(quartile).Student's t test or Wilcoxon T test was performed to analyze the significance differences of the paired and unpaired continuous variables.Pearson ?2or Fisher's exact test was conducted to analyze the expression or distribution differences of the variables.Kaplan-Meier method and multivariate Cox proportional hazard regression analysis was used to estimate the potential prognosis associated indicators.P-values were two sides,and P < 0.05 was considered statistically significant in all tests.Results: The expression of Linc01021 in colorectal cancer tissues was significantly down-regulated compare with the paired tumor-adjacent normal controls,and the median positive expression rates were 40% and 35%,respectively(P <0.05).Meanwhile,the expression levels of mi R-576 and mi R-425 were significantly up-regulated in colorectal cancer tissues-(median expression rates:15% and 47%;12% and 33%,respectively).The linear correlation analysis confirmed that the Linc01021 expression were negative correlated with mi R-576 and mi R-425 expression,whereas mi R-576 expression was positive correlated with mi R-425 expression.Furthermore,the clinicopathological parameters correlation analysis demonstrated that Linc01021 high expression was significantly associated with the the primary location [P = 0.011,OR = 0.385(0.184-0.804)] and tumor size [P = 0.011,OR = 3.575(1.630-7.843)].The mi R-576 high expression was remarkably related to the intestinal perimeter [P = 0.015,OR = 0.379(0.173-0.830)] and the high expression level of mi R-425 showed significantly related to tumor size [ P = 0.004,OR = 0.340(0.163-0.710)].For colorectal cancer patients who receiving oxaliplatin-based chemotherapy,Linc01021 high expression had a better disease-free survival(DFS),and the MST of high and low-expressing were 58 months and 39 months,respectively.Furthermore,the overall survival(OS)were also prolonged in this cohorts(62 months and 42 months for high or low expression MST of Linc01021,respectively).Moreover,patients with higher expression levels of mi R-576 and mi R-425 had a worse DFS(mi R-576: MST = 36 months and 58 months;mi R-425: MST = 37 months and 58 months for high or low expression,respectively)and OS(mi R-576: MST = 39 months and 63 months;mi R-425: MST = 40 months and 62 months for high or low expression,respectively)in these patients treated with oxaliplatin-based chemotherapy.Further prognostic multivariate COX regression analysis further revealed that Linc01021 was a protective factor for the prognosis of colorectal cancer patients [DFS and OS are: P = 0.009 and 0.011,HR = 0.577(0.382-0.870)and 0.573(0.372)-0.882)].In contrast,mi R-576 [DFS and OF are: P = 0.009 and 0.011,HR = 1.992(1.331-2.981)and 2.071(1.357-3.160)] and mi R-425 [DFS and OF are: P = 0.001 and 0.002,HR = 1.934(1.293-2.895)and 1.924(1.260-2.938)] are risk factors for the prognosis of colorectal cancer patients.Additionally,the results of stratificated analysis further indicated that the high expression of Linc01021 and the low expression of mi R-576/mi R-576 were more effective protective factors for the survival time of the colorectal cancer patients.Conclusion: In colorectal cancer tissues,the down-regulated expression of Linc01021 were detected accompanied with the up-regulated expression of mi R-576 and mi R-425.The expression levels of Linc01021 showed negatively correlated with the expression of the mi R-576 and mi R-425.In this included cohort,the Linc01021 high expression levels are significantly associated with the primary sites and the tumor size,meanwhile the mi R-576 high expression related with the intestinal perimeter and mi R-425 high expression correlate with the tumor size.Finally,the Linc01021 acts as a protective prognostic factor which prolonged the DFS and OS survival times.In contrast,mi R-576 and mi R-425 exerts their risk factors for a worse survival time for those patients with oxaliplatin-based chemotherapy. |
PDF Full Text Request