Metabolomic Changes Of Severe Community-acquired Pneumonia Caused By Different Pathogens In Children

Purpose:Metabolomics was used to analyze the differences in serum metabolic profiles of children with severe communityacquired pneumonia caused by different pathogens in order to find the relevant differences in metabolites and metabolic pathways Method:1.Collect the frozen blood samples of children admitted to the intensive medicine department(PICU)Hunan Provincial People's Hospital from January 2017 to February 2019,collect demographic data,clinical data and laboratory examination,etc.,and group according to the results of etiological examination.2.Quantitative analysis of metabolites in residual serum of children with severe community-acquired pneumonia caused by different pathogenic infections using metabolomics techniques.principal component analysis(PCA)and orthogonal partial least squares(OPLS-DA)multivariate statistical analysis yielded differential weight contribution(VIP)values.major differential metabolites were obtained by VIP>1 and t tests(P>0.05).analysis of differential metabolites through existing databases to find differential metabolic pathways.Result:1.There were 33 children with severe community-acquired pneumonia(community-acquired pneumonia,CAP),including 10 in mycoplasma pneumoniae(mycoplasma pneumoniae,MP)infection group,13 in human adenovirus(human advenovirus,HAdV)infection group and 10 in respiratory syncytial virus(respiratory syncytial virus,RSV)infection group.Example.The male/female ratio was 1.06:1(17 males and 16 females).Age distribution of selected children was consistent with the clinical prevalence of mycoplasma pneumoniae infection in preschool children,and human adenovirus,respiratory syncytial virus infection was common in children aged 2~ June.the mean age±standard deviation of children in MP infection group was 52±5.1 months.the mean age±standard deviation of children in HAdV infection group was 26±2.1 months.the mean±standard deviation of children in RSV infection group was 13±2.0 months.2.By QC quality control,data normalization results confirmed that this study data reliable.3.There were significant metabolic differences between MP infection group and HAdV infection group,MP infection group and RSV infection group.4.Metabolic differential identification :The serum differential metabolites of MP infection group and HAdV infection group included 22 species,including ?-linolenic acid,glycine,tryptophan,linoleic carnitine,pyruvate,androstenedione.serum differential metabolites in the MP and RSV infection groups included 19 species,including Lhistidine,glycine,p-hydroxybutyric acid,and oleyl carnitine.serum differential metabolites in the HAd V and RSV infection groups included 15 species,including L-histidine,N-lactose-based tryptophan and sulfonic acid.5.Metabolic pathways involved in metabolic differences: serum differential metabolites in the MP and HAdV infection groups involved metabolic pathways glycine,serine and threonine metabolism,gluconeogenesis/glycemia.serum differential metabolites in MP and RSV infected groups involved metabolic pathways involving histidine metabolism,glycine,serine and threonine metabolism.the main serum differential metabolites in HAdV and RSV infection groups involved metabolic pathway pathways,such as glutathione metabolism,glycine,serine and threonine metabolism.6.The differential metabolites in MP infection group,HAdV infection group and RSV infection group were all associated with glycine,threonine and serine metabolic pathways.Conclusion: There were differences in serum metabolites between the MP infection group,the HAdV infection group and the RSV infection group,and the differential metabolites involved metabolic pathways were common in glycine,threonine and serine metabolic pathways.