| Objective:To investigate the expression and clinical significance of AP1S1(AP-1 complex subunit sigma-1A)gene in breast cancer.Methods:The relationship between the expression level of AP1S1 and the clinical prognosis of breast cancer patients was analyzed using the breast cancer case data published in GEPIA and UALCAN online databases.110 cases of wax block specimens with complete clinical data and complete clinical data were collected from the provincial hospital affiliated to Anhui Medical University from 2005 to 2016.The expression of AP1S1 in breast cancer tissues was detected by immunohistochemistry.In vitro experiments were performed using plate clones,CCK8 and cell scratch experiments to analyze the effect of AP1S1 expression on the proliferation and metastasis characteristics of breast cancer cells.Results:Through the analysis of GEPIA and UALCAN data,it was found that the expression level of AP1S1 in breast cancer tissues was significantly higher than that in normal breast tissues(P <0.01).The expression level of AP1S1 was significantly associated with overall survival(OS)and disease-free survival(DFS)(P <0.05),and it is closely related to the tumor stage of breast cancer patients(P <0.05),and has no obvious correlation with age,tumor classification and lymph node metastasis.Clinical case analysis confirmed that AP1S1 expression was closely related to breast cancer tumor size and AJCC stage(P <0.05),and had no significant correlation with age,lymph node metastasis,ER status,PR status,and HER2 status(P> 0.05).The results of cell function experiments showed that the expression of AP1S1 gene can promote the proliferation,colony formation and migration ability of breast cancer cells.Conclusion AP1S1 gene is highly expressed in breast cancer tissues,and its expression level is related to the prognosis of breast cancer patients.Moreover,AP1S1 gene expression can promote breast cancer cell proliferation,colony formation and migration ability. |
