Expression And Clinical Significance Of UDO-glucose 6-dehydrogenase In Pancreatic Cancer

Objective: To explore the relationship between the expression of UGDH gene in cancer tissues and adjacent tissues,clinical characteristics and prognosis in patients with pancreatic cancer.Methods: Bioinformatics method was used to analyze the expression of UGDH genes in pancreatic cancer and the UGDH gene and its co-expressed genes were analyzed by GO and KEGG pathway.The relationship between UGDH expression and the clinical area group of pancreatic cancer was analyzed,and the relationship between UGDH expression and survival prognosis of pancreatic cancer patients was analyzed.The methylation of UGDH gene in pancreatic cancer was detected,CNV and SNP analysis,PPI prediction and GSEA analysis of UGDH gene were performed,and the upstream regulation of mi RNA was predicted.Immunohistochemical staining was used to detect the expression of UGDH in pancreatic cancer tissues and adjacent tissues,and the relationship between UGDH gene expression and clinicopathological parameters and prognosis was analyzed in combination with clinicopathological data.Results: Bioinformatics analysis showed that UGDH gene was highly expressed in pancreatic cancer.Analysis of the clinical area group showed that only the history of drinking and the tumor site were closely related to the expression level of UGDH.(P<0.05).Compared with the low-expression group,UGDH patients with pancreatic cancer had significantly lower overall survival rate and shorter survival period(P<0.05).GO and KEGG pathway analysis and GSEA showed that genes significantly co-expressed with UGDH were enriched into a number of cancer-related biological functions and signaling pathways,including pancreatic cancer pathways.In pancreatic cancer tumor samples,the methylation level of UGDH gene promoter region was significantly higher than that of UGDH gene promoter region in normal tissues.In the TCGA database,SNP statistics showed that the mutation frequency of UGDH gene in pancreatic cancer was 1%.The incidence of CNV events in pancreatic cancer with UGDH was 2.26%.PPI prediction and key subnetwork analysis showed that HGS,UBE2V1,MAT2 A and SUMO1 played an important role in the interaction network,and their expressions were significantly positively correlated with UGDH gene expression.Functional and pathway enrichment analysis revealed that the genes in the network were concentrated in protein localization in membrane,biosynthesis of carbohydrate derivatives,regulation of protein catabolism,modification of peptide lysine,and the second stage-compound binding pathway.The results of clinical samples showed that the UGDH positive expression rate in pancreatic cancer tissues was significantly higher than that in adjacent tissues,and the UGDH expression level in pancreatic cancer tissues was significantly higher than that in adjacent tissues,and the difference was statistically significant(P < 0.05).The positive expression of UGDH was associated with the history of chronic pancreatitis(P=0.024),lymph node metastasis(P=0.003)and nerve invasion(P=0.037)in patients with pancreatic cancer.The survival analysis showed that the prognosis of pancreatic cancer patients in UGDH positive expression group was worse than that in the negative expression group.Conclusion: 1.Comprehensive bioinformatics analysis showed that UGDH was highly expressed in pancreatic cancer,and its expression may be closely related to the occurrence,biological function and poor prognosis of pancreatic cancer.UGDH expression is not dependent on gene mutation,and may be regulated by mi RNA.2.UGDH expression and clinical prognosis analysis of pancreatic cancer showed that UGDH expression in pancreatic cancer was significantly higher than that in adjacent tissues,and its high expression could be used as an independent risk factor for poor prognosis of pancreatic cancer.3.UGDH is expected to become a potential new target for the prediction and treatment of pancreatic cancer.