Study On Metabolic Varieties Of Intestinal Microbiota In Infants With Dyschezia

Objective:By analyzing the varieties of short chain fatty acid(SCFA)produced by the intestinal microbiota in infant dyschezia(fecal retention),we try to discuss the metabolic characteristics of intestinal microbiota in infants with dyschezia.The present results will provide constructive inspiration on the pathogenesis of infant dyschezia and useful suggestions for the future clinical intervention.Materials and methods:Twenty-one infants with a clear diagnosis of infant dyschezia in the outpatient department affiliated Children’s Hospital of Soochow University and Suzhou Municipal Hospital from April 2019 to October 2019 were collected and designated as observation group.They were ten males and eleven females,aged from 3 to 9 months old,with an average of 4.53 months.Twenty-four healthy infants in department of child care,Children’s Hospital of Soochow University were collected and designated as control group.They were eleven males and thirteen females,aged from 3 to 9 months old,with an average of 4.24 months.Fresh fecal samples were collected within 2 hours after being excreted from the two groups of subjects,and they were stored in dry,non-absorbable,sterile,and sealed containers after sampling.Appropriate amount of sample were weighed and placed it in a stool analyzer,then added PBS buffer to make 10%fecal suspension.Then fresh fecal suspension were inoculated into the anaerobic bottles contained basic medium(YCFA),or 8g/L of lactulose(LAU),fructooligosaccharide(FOS),galactooligosaccharide(GOS),inulin(INU),soluble starch(STA),mannitol(MAI),and incubated at 37℃ for 24 hours.The gas production in the various media was detected by gas pressure measuring instrument.The concentration of total SCFA,acetate,propionate,butyrate,isovalerate,valerate and isobutyrate in those media were detected by Gas chromatography(GC).SPSS 23.0 software was used to analyze the data.Results:1.The concentration of propionate in observation group was significantly higher than that of the control group(P<0.05).The concentration of isovalerate in observation group was significantly lower than that of the control group(P<0.05).There was no statistical significance in concentration of total SCFA,acetate and butyrate in feces of both groups.2.The molar ratio of acetate:propionate:butyrate in the total SCFA was 77.2:17.2:2.7 in feces of observation group and 85.9:9.7:2.1 in control group.The proportion of propionate in observation group was significantly higher than that of control group(P<0.05).3.The total SCFA concentration in YCFA,LAU,FOS,GOS,INU,STA media in the observation group was significantly higher than that in control group after fermentation(P<0.05),and the results in the MAI medium were not statistically significant.4.The concentration of acetate in YCFA,LAU,FOS,GOS,INU,STA media in the observation group was significantly higher than that in control group after fermentation(P<0.05),and the results in the MAI medium were not statistically significant.5.The concentration of propionate in YCFA,LAU,FOS,GOS,STA,MAI media in the observation group was significantly higher than that in control group after fermentation(P<0.05),and the results in the INU medium were not statistically significant.6.There was no statistical significance in butyrate concentration between the observation group and the control group after fermentation in all testing media(P>0.05).7.The concentration of isovalerate in YCFA,LAU,FOS,GOS,INU,STA,MAI media in the observation group was significantly lower than that in control group after fermentation(P<0.05).8.The gas production in MAI media in the observation group was significantly less than that of control group after fermentation(P<0.05),and there was no statistical significance in the rest of the media(P>0.05).Conclusion:1.SCFA is the main production of intestinal microbiota metabolism in infants,and the molar ratio of acetate,propionate and butyrate in total SCFA was 85.9:9.7:2.1.2.The concentration of propionate producted by the intestinal microbiota of infants with dyschezia is higher than that of normal infants,and the molar ratio of acetate,propionate and butyrate in total SCFA was 77.2:17.2:2.7.Current data suggests that the infant with dyschezia has the abnormal development of intestinal microbiota.The change of SCFA composition and the adulthood trend of intestinal microbiota in the infants indirectly confirms the involvement of intestinal microbiota in the causative mechanism of infant dyschezia.3.The molar ratio and total concentration of acetate,propionate,butyrate produced by the intestinal microbiota of infants with dyschezia is imbalanced,which may cause colon motility disorders and further affect the coordination of defecation.4.The ability of fermenting oligosaccharides to produce SCFA was enhanced in infants with dyschezia,especially the increase in the production of acetate and propionate.The increased concentration of acetate and propionate may inhibit the peristalsis of the immature colon.5.Current data suggested the potential application of probiotics to interfere with infant dyschezia.6.Determination of SCFA in the in vitro fermentation model can be used as a simple and useful approach to evaluate the activity of intestinal microbiota and its metabolic changes in infants with dyschezia.