Study On Effects And Mechanism Of Cerium Oxide Nanoparticles In Sepsis

Objective:The effects and mechanism of CeO2NPs on sepsis were studied by respectively administering CeO2NPs to rats and children with sepsis and testing TNF-? in Kupffer cells from rats,TNF-? in macrophages from peripheral blood of children and CeO2 levels in liver cells from rats.Methods:1.Black grape juice was extracted to synthesize CeO2NPs by gel-sol method,and then CeO2NPs were characterized via fourier transform infrared reflection,X-ray diffraction and transmission electron microscope.2.Rats in each group were administered separately.24 hours later,kupffer cells were isolated and cultured,and liver tissue sections were stained.3.Levels of inflammatory factor TNF-? in kupffer cells from rats in each group were ELISA-assayed and compared.4.CeO2 levels in the liver were assayed via ICP-MS,and histopathological examination were measured.5.60 samples of 2 ml of peripheral blood were collected in total from children with sepsis.6.Mononuclear cells were extracted from peripheral blood and induced into macrophages for culture.7.Levels of inflammatory factor TNF-? in macrophages of children in each group were ELISA-assayed and compared.Results:1.By gel-sol method,light yellow CeO2NPs were successfully synthesized around 267mm in UV spectrum.They are 37± 2nm spherical agglomerated particles composed of cerium and oxygen with high stability.2.As compared with the control group,when grape extracts and CeO2NPs were injected through the tail vein and when they were both injected intravenously,there was no significant difference in the levels of TNF-? released by kupffer cells;when induced by LPS injection through the tail vein,the levels of TNF-? released significantly increased;but when LPS and CeO2NPs were injected intravenously,the levels of TNF-? released were significantly reduced,with the difference was statistically significant(P<0.05).3.As compared with the control group,when CeO2NPs were injected through the tail vein or LPS and CeO2NPs were injected intravenously at the same time,the level of CeO2 assayed in liver significantly increased,with the difference was statistically significant(P<0.05).As compared with the control group,when induced by LPS injection through the tail vein,liver cells showed swelling,necrosis and other related changes;but when LPS and CeO2NPs were injected intravenously,the changes of liver cells were diminished.4.As compared with the control group,the levels of TNF-? released by macrophages in peripheral blood of LPS-induced children significantly increased,with the difference statistically significant(P<0.05).5.When LPS induced,the level of TNF-? released significantly increased;but when LPS and CeO2NPs were injected intravenously at the same time,the level of TNF-?released was significantly reduced,with the difference statistically significant(P<0.05).Conclusions:1.CeO2NPs can inhibit the release of TNF-? in LPS-induced rats with sepsis.2.CeO2NPs can increase the level of CeO2 in liver and protect the liver cells against sepsis.3.CeO2NPs can inhibit the release of TNF-? in children with sepsis.