Correlation Between The Abnormal Expression Of MiR-711 And The Occurrence Of Ulcerative Colitis In Mice

Objective And Background: Ulcerative colitis(UC)is a chronic inflammatory bowel disease.It starts with rectal mucosal inflammation and extends proximally in a continuous manner.The incidence of UC has increased significantly in recent years,but its pathogenesis is not clear,the treatment effect is not good,and it seriously affects the quality of life of patients.When reviewing the literature,we found that miR-711 is related to its inflammatory response after organ and tissue damage,and there may be a correlation between miR-711 and ulcerative colitis,but no research has been reported.In this study,we explored the differences in the expression levels of miR-711 in mouse models of ulcerative colitis to provide a basis for finding new biomarkers for ulcerative colitis.Methods: In this experiment,dextran sulphate sodium(DSS)was used to build a mouse ulcerative colitis model.Sixteen C57 BL / 6 male mice were randomly divided into experimental group 10 and control group 6.The mice in the experimental group were free to drink 3% dextran sodium sulfate(DSS)for 7 consecutive days.Observing the weight,Occult blood in stool,and stool characteristics of the mice were observed daily and DAI scores were obtained.By assessing the weight,stool characteristics,occult blood,and histopathology of the colon,We judge that the ulcerative colitis model has been successfully established.At the same time,mice in the control group drink distilled water freely.After seven days,mice were sacrificed by neck-breaking.Collecting colonic tissue of each mouse.Colonic issues were used for HE staining to evaluate pathological conditions and extracting RNA.Reverse transcription into cDNA was performed.The expression of miR-711 in the experimental and control groups was detected by real-time fluorescence quantitative PCR,and the difference of content was compared between the two groups.Results: Four of the ten mice in the experimental group had no obvious symptoms such as blood in the stool,diarrhea,weight loss,and no significant pathological changes.Therefore,it was judged that they were not successfully induced and were eliminated.The remaining 6 mice in the experimental group were compared with the normal control group,the mice in the experimental group showed obvious symptoms of malaise,weight loss,diarrhea and blood in the stool,and the DAI scores of the mice gradually increased;the mice in the experimental group(remove 4unsuccessful mice)had congestion,swelling and shortening of the colon.The results of HE staining showed that the colonic epithelial cells and glands of the experimental group were damaged,the crypt structure was deformed,a large number of inflammatory cells were infiltrated,and the villi were arranged disorderly.At the end of modeling,The weight of the mice in the experimental group(excluding the 4 mice without successful induction)was(17.55?0.88)g,DAI score was(3.16?0.18),and the colorectal length was(5.13 ? 0.73)cm.The weight of the mice in the control group was(22.94 ? 0.97)g,DAI score was 0,and the colorectal length was(8.74?0.44)cm.The differences between the two groups were statistically significant(P <0.05).The results of qRT-PCR(2-??Ct)showed that miR-711 was lower in the colonic tissue of ulcerative colitis mice than the control group(P <0.05).Conclusion: The content of miR-711 in ulcerative colitis mice is significantly lower(P <0.05),which suggest that miR-711 may have an effect on the occurrence and development of ulcerative colitis.