Effect Of Triptolide On The Proliferation Of Human Retinal Capillary Endothelial Cells And The Expression Of VEGF In High Glucose Condition

Purpose:To study the effects of different concentrations of triptolide on the proliferation of human retinal capillary endothelial cells(HRCECs)and vascular endothelial growth factor(VEGF)expression under high glucose conditions in vitro.Methods:1.HRCECs extracted from donated human eyes were cultured in vitro,and well-developed third to fourth generation cells were used for experiments.2.The thiazole blue colorimetric method(MTT)was used to detect the proliferation of HRCECs cultured in vitro under high glucose environments under the action of different concentrations of triptolide.3.Western blot method was used to detect the VEGF content of HRCECs in each group.Results:The results of MTT colorimetry showed that the proliferation of HRCECs was inhibited to varying degrees through the intervention of triptolide at different concentrations on HRCECs cultured in a high glucose environment.The specific performance is as follows:1.Under the same culture time,the number of HRCECs in the low glucose group is significantly less than that in the high glucose group,P <0.05,which is statistically significant;2.Under the same culture time(24h,36 h,48h),the high glucose group was compared to another high glucose group with different concentrations of triptolide within a certain concentration range(20nmol/l,40nmol/,60nmol/),the inhibition rate of HRCECs increased with the increase of triptolide concentration;3.Under the intervention of the same triptolide concentration,two pairs of comparisons were conducted at different time points.Within a certain time range,the inhibition rate of HRCECs showed an increasing trend which correlated with the extension of the intervention time;4.Under the same culture time(24h,36 h,48h),there was no statistically significant difference in absorbance between the high glucose group and the high glucose with solvent control group,P >0.05,no statistical significance.Western blot showed:1.Under the same culture time,the expression levels of VEGF in the low glucose group HRCECs of the contrast protein band were significantly lower than those in the high glucose group,P <0.05,which was statistically significant;2.At the same incubation time(24h,36 h,48h),the high glucose group and the solvent control group,by comparing the gray value of protein bands,there was no significant difference in the expression of VEGF in HRCECs of the two groups,P> 0.05,which was statistically significant;3.The high glucose group was compared to another high glucose group with different concentrations of triptolide(20nmol/l,40nmol/,60nmol/),the two groups were compared in pairs.As the triptolide concentration increased,the expression of VEGF in the gray value HRCECs of the contrast protein bands showed a downward trend,P <0.05,which was statistically significant;4.Under the intervention of the same triptolide concentration,a pairwise comparison was made at different time points.Within a certain time range,the expression of VEGF in the gray value of the contrasted protein band HRCECs showed a downward trend which correlated with the extension of the intervention time,P <0.05,which was statistically significant.Conclusion:1.Triptolide has an inhibitive effect on the proliferation of in vitro HRCECs in high glucose environments,depending on time and triptolide concentration;2.The expression of VEGF in HRCECs was inhibited within a certain range of triptolide intervention time and concentration.