| BackgroundType 2 diabetes is a metabolic disease characterized by a chronic low-grade inflammatory state.Recent studies have shown that the development of type 2 diabetes is closely related to the intestinal flora.Intestinal flora is the largest microecosystem in the human body,which is involved in the metabolism of substances and energy of the body.Genetic factors,diet,antibiotics and other factors can cause changes in intestinal flora.Some studies have shown that the imbalance of intestinal flora may have a significant impact on the prognosis of patients with type 2 diabetes.Antibiotics are widely used in clinic.Therefore,it is of great clinical significance to observe the effect of antibiotics on intestinal microecology of type 2 diabetes mellitus with antibiotics as the intervention means and intestinal flora as the target.ObjectiveThe effect of ceftriaxone sodium on intestinal flora,intestinal mucosa and expression of inflammatory factors in rats with type 2 diabetes was observed by constructing a type 2diabetic rat model and intervening with ceftriaxone sodium.Methods40 healthy male SD rats were randomly selected for the normal group,and the remaining 35 rats were given high-fat and high-sugar combined with streptozotocin to construct the type 2 diabetic rat model,and 5 non-model rats were excluded.From 30 ChengMo rats randomly selected 5 for diabetes group,the remaining 25 only by intraperitoneal injection of ceftriaxone sodium 5 consecutive days,respectively,in the use of ceftriaxone sodium 1,3,5 days and disable ceftriaxone sodium 1,2 weeks eachrandomly selected five rats,normal group and diabetes group such as weight than physiological saline injections.The changes of intestinal flora were observed by ERIC-PCR and bacterial culture,the pathological changes of jejunum mucosa were observed by HE staining,and the expression of IL-1β in jejunum was detected by immunohistochemistry.Results1.ERIC-PCR fingerprint: Compared with the diabetes group,the diversity index of ceftriaxone sodium group on day 5 decreased,with statistically significant difference(P <0.05).The diversity index of ceftriaxone sodium group at week 2 was higher than that of ceftriaxone sodium group at day 5,and the difference was statistically significant(P <0.05).Similarity clustering analysis showed that the difference between the ceftriaxone sodium group on day 5 and the diabetes group was significant,and the difference between the ceftriaxone sodium sodium group on week 2 was similar to the diabetes group,but there was some difference.2.Intestinal colony count:The number of bifidobacteria,lactobacillus,proteus and escherichia coli in the ceftriaxone sodium group on day 5 was lower than that in the diabetes group,and the difference was statistically significant(P < 0.05).The number of four bacterial colonies in the group at week 2 of ceftriaxone sodium was increased compared with the group at day 5 of ceftriaxone sodium,and the difference was statistically significant(P < 0.05).3.Pathology of jejunal mucosa: In the diabetes group,the villi were irregularly arranged,with fracture Spaces and mucosal hierarchy disorder.On the 5th day of ceftriaxone sodium,the villi in the group were sparse,swollen and detached,with irregular shape.The subepithelial space at the top of the villi was widened,and a large number of lymphocytes were infiltrated in the submucosa.The recovery of intestinal mucosa in the second week of ceftriaxone sodium was close to that in the diabetic group.4.Jejunum inflammatory factor expression: Compared with the diabetes group,IL-1βwas increased in the ceftriaxone sodium group on day 5,and the difference was statisticallysignificant(P < 0.05).Compared with ceftriaxone sodium group on day 5,IL-1β’s expression was decreased in the group at week 2,and the difference was statistically significant(P < 0.05).Conclusion1.The use of ceftriaxone sodium can lead to the decrease of intestinal flora diversity in rats with type 2 diabetes,leading to the imbalance of intestinal flora.2.The maladjustment of intestinal flora in rats with type 2 diabetes may lead to changes in intestinal microecology,which may damage the intestinal mucosal barrier and promote the expression of inflammatory factors,possibly increasing the risk of enterogenous infection. |
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