Expressions And Clinical Significance Of TLR-3,CD80 And NF-?B In Renal Tissue Of Children With Minimal Change Nephropathy

BackgroundMinimal change nephropathy(MCN)is the most common type of primary nephrotic syndrome(PNS)in childhood,and its pathogenesis is still unclear,It may be related to T lymphocyte dysfunction,In recent years,it has been found that the toll like receptors(TLRs)signaling pathway can be involved in a variety of infectious and non infectious kidney diseases.We found that the expression level of TLRs in kidney tissue and peripheral blood mononuclear cells of children with PNS may be related to pathological type and disease activity.At present,there is little research on TLR-3 expression in renal tissue of PNS children.It has been found that TLR-3 ligands can induce CD80 to express on cultured renal podocytes through nuclear factor-kappa B(NF-?B)signaling pathway,leading to proteinuria.In this study,the expression of TLR-3,CD80,NF-?B in renal tissue of PNS children diagnosed as MCN by renal biopsy was detected by immunohistochemistry,and the relationship between TLR-3,CD80,NF-?B and related clinical test indexes before renal biopsy will be analyzed,and the possible role of TLR-3,CD80,NF-?B and relation to clinical test indexes before renal puncture in the pathogenesis of MCN in children will be discussed.ObjectiveThe expressions levels of TLR-3,CD80 and NF-?B in renal tissue of MCN,the most common pathological type of PNS in children,were detected,and the relationship between TLR-3,CD80,NF-?B and the clinical examination indexes before renal biopsy was analyzed,To explore the possible role of TLR-3,CD80,NF-?B and the clinical examination indexes before renal puncture in the pathogenesis of MCN in children.Methods29 PNS children(18 males,11 females,aged < 18 years old)with MCN confirmed by renal biopsy at the first affiliated hospital of Xinxiang Medical University from October 2017 to September 2019 were included in the study.Normal kidney tissues of 5 children(2 males and 3 females)with nephroblastoma were used as a control group(these kidney tissues away from the nephroblastoma tissue was confirmed by light microscopy)in the Department of Pediatric Surgery,the First Affiliated Hospital of Xinxiang Medical University.The expression of TLR-3,CD80 and NF-?B in renal tissue was evaluated by immunohistochemistry,and the levels of 24-hour urine protein,total cholesterol,albumin,creatinine and urea nitrogen before renal biopsy were measured by spectrophotometry.ResultsThe expression of TLR-3 in renal tissue cells is nucleus expression,while CD80 and NF-?B are expressed in renal tissue cells cytoplasm.Control group: There is a small amount of TLR-3 expression in renal tubular epithelial cells,while there is almost no TLR-3 expression in the glomeruli.CD80 and NF-?B were slightly expressed in renal tubular epithelial cells and glomerular mesangial cells.In MCN group,TLR-3 expression was almost absent in renal tubular epithelial cells and glomerulus;CD80 and NF-?B were highly expressed in renal intrinsic cells(mainly renal tubular epithelial cells and mesangial cells),the expression of renal tubular epithelial cells was dominated by distal renal tubular epithelial cells.The expressions of CD80 and NF-?B in the MCN group were(0.14±0.06)and(0.20±0.06),respectively,which were higher than those of CD80(0.05±0.05)and NF-?B(0.14±0.06)in the control group,with statistically significant differences(P<0.05).The expressions of NF-?B and CD80 were positively correlated(P<0.05),and NF-?B,CD80 were positively correlated with 24-hour urine protein(P all<0.05).Conclusion1.The abnormal activation of CD80 and NF-?B may be involved in the pathogenesis of MCN;2.TLR-3 was not expressed in MCN group,suggesting that CD80 and NF-?B may be activated by other signaling pathways;3.24-hour urinary protein is a potential injury or risk factor for MCN progression.