Expression And Significance Of Zinc Finger Protein A20in Peripheral Blood Of Patients With Hypothyroidism

Objective: Typothyroidism is a very common disease with complex etiology,but autoimmune thyroiditis is the most common cause of hypothyroidism.Many studies have shown that zinc finger protein A20 plays a key role in the negative regulation of inflammation and immunity,and participates in the pathogenesis of many autoimmune diseases.In this study,the relationship between zinc finger protein A20 and the development of hypothyroidism was studied by detecting the level of zinc finger protein A20 in peripheral blood mononuclear cells of patients with hypothyroidism.Methods:(1)The expression of zinc finger protein A20 mRNA in peripheral blood mononuclear cells(PBMC)of 50 hypothyroid patients and 50 healthy people was detected by Quantitative Real-time PCR(Real-time PCR).(2)The relative expression level of zinc finger protein A20 mRNA in peripheral blood mononuclear cells of hypothyroidism group was correlated with the common clinical indicators of autoimmune thyroiditis,TGAb and TPOAb.Results: Real time PCR showed that the relative expression level of serum zinc finger protein A20 in PBMC of hypothyroidism patients was 0.9264 ± 0.3465,and that in normal control group was 1.2077 ± 0.3534;compared with the two groups,the expression level of zinc finger protein A20 in hypothyroidism group was significantly lower than that in normal control group(P < 0.05).Conclusion: The expression of zinc finger protein a20 mrna in peripheral blood mononuclear cells of hypothyroidism patients is low,which is related to the common clinical indicators of autoimmune thyroiditis.It suggests that zinc finger protein A20 participates in the occurrence and development of hypothyroidism,and reflects the change of thyroid function to a certain extent.It is of great significance for early diagnosis of hypothyroidism to monitor the changes of peripheral zinc finger protein A20.