Experimental Study On The Effect Of Nourishing Yin,Strengthening Spleen And Dispersing Toxin Prescription On AKT Signal Pathway Of Human Gastric Cancer SGC-7901 Cells

Objective:1.In this experiment,the effects of Nourishing Yin,strengthening spleen and dispersing toxin prescription on the proliferation,migration,invasion,apoptosis,and cycle of gastric cancer SGC-7901 cells were observed,and the AKT signal pathway and related factors in the cells were detected after Chinese medicine intervention Protein expression level.2.Compare the effects of formula Ⅰ and formula Ⅱ on gastric cancer cells,study the molecular signaling pathway mechanism of this formula in the treatment of advanced gastric cancer,discuss its scientific theoretical basis,summarize its treatment characteristics,and provide optimization for traditional Chinese medicine treatment and clinical application in accordance with..Method:Experimental study:The water extracts of Nourishing Yin,strengthening spleen and dispersing toxin prescription(No.Ⅰ and No.Ⅱ)were prepared,and the gastric cancer SGC-7901 cells were cultured in a cell incubator.Effects of SGC-7901 cell proliferation level,Transwell chamber migration method to detect changes in cell invasion and migration ability,flow cytometry Annexin V-FITC/PI double staining method to detect changes in apoptosis level,Western Blot test to detect Yangyin Jianpi San The expression levels of the four proteins akt,p-akt,Bcl-2,and Bax in the two cells after the intervention of water extract.Results:1.The CCK8 experiment results show that with the increase of drug concentration(1%,2%,3%,4%,5%)and time(24h,48h,72h),the proliferation rate of SGC-7901 cells was gradually increased,and it had significant statistical significance compared with the control group(P<0.01).Calculated by SPSS 26.0,when the medication time is 48 hours,IC Ⅰ=1.543%and ICⅡ=4.953%;2.The results of Transwell experiment show that compared with the control group,it has significant statistical significance(P<0.01).The experimental group(Part Ⅰ,Part Ⅱ)can inhibit the migration and invasion levels of SGC-7901 cells.Time-concentration dependent;3.Flow cytometry Annexin V-FITC/PI double staining results showed that the gastric cancer SGC-7901 cells had a significantly increased apoptosis rate 48 hours after the experimental group(No.Ⅰ and No.Ⅱ).And concentration-dependent;4..Western Blot results showed that compared with the control group,the expression levels of akt,p-akt,and Bcl-2 in the experimental group(No.Ⅰ and No.Ⅱ)decreased,while the expression of Bax increased significantly(P<0.05).Conclusion:Nourishing Yin,strengthening spleen and dispersing toxin prescription can inhibit the proliferation,migration and invasion of gastric cancer SGC-7901 cells,and induce its apoptosis.Its antitumor mechanism may be related to its intervention in the levels of akt,p-akt,Bax,Bcl-2 proteins.Expression related.