Clinical Significance And Biological Function Of IFIT2 Expression In Human Non-small-cell Lung Cancer

Objective IFIT2 is an important interferon-stimulated gene family protein,which has been proven to play an important role in the process of anti-tumor and anti-virus.The purpose of this study was to investigate the IFIT2 expression in human non-small-cell cancer(NSCLC)tissues and its clinical implications,as well as the biological function of IFIT2.Methods The immunohistochemistry assay was used to identify the clinical significance and prognostic value of IFIT2 expression in NSCLC tissues.The depletion of IFIT2 was achieved using RNAi approach.Real-time PCR and Western Blot were used to detect the IFT2 expression in the cell line Lv-shIFIT2 group at the RNA and the protein level,respectively.CCK-8 proliferation assay,Wound healing assey and Transwell invation assay were used to study the role of IFIT2 in NSCLC cell biological behaviors.Results Decreased IFIT2 expression was found in human NSCLC tissues(both in lung adenocarcinoma and lung squamous cell carcinoma)in contrast to the adjacent normal tissues(both P<0.0001 respectively).We did not find any significant correlations between the IFIT2 expression and patient's clinicopathological features.The survival analysis showed that the overall survival(OS)of patients in IFIT2 low expression group was significantly poorer than that in IFIT2 high expression group(in lung adenocarcinoma:P=0.027;and in lung squamous cell carcinoma:P=0.029).The Cox model analysis also indicated that the distant metastasis(P=0.043)could be used as an independent prognostic factor for lung adenocarcinoma patients,and the lymph node metastasis(P=0.045)and IFIT2 low expression(P=0.020)could be used as independent prognostic factors for lung squamous cell carcinoma patients.Moreover,the depletion of IFIT2 in human lung cancer cell lines A549,H1975 and SK-MES-1 significantly increased the cellular abilities,such as viability,migration and invasion.Conclusion The study have shown that the decreased IFIT2 was involved in the occurrence and development of lung cancer and closely related to the prognosis of lung cancer patients.The IFIT2 down-regulating significantly enhanced the proliferation,migration,and invasion of NSCLC cells.