Differentially Expressed MRNA Can Be Used As A Potential Marker For Tumor Prognosis In Papillary Renal Cell Carcinoma

BackgroundThe clear cell renal cell carcinoma(ccRCC)is the most common pathological type of renal cell carcinoma.In the past the research and development of renal cancer drugs,by domestic and foreign scientific research institutions and pharmaceutical companies,had mainly focused on clear cell carcinoma.Papillary renal cell carcinoma(pRCC)is the second most common pathological type of renal cancer,accounting for about 10%-15%of renal cell carcinoma.Various targeted drugs commonly used in clinical practice are mainly developed for clear cell renal cell carcinoma,and there is no targeted drug specifically for papillary renal cell carcinoma.At present,clinical research has entered the era of high-throughput sequencing.By sequencing genes in cancer tissues and adjacent tissues,it was found that genes compared with normal tissues were higher or lower expression in cancer tissues.By screening the effective gene pathways,we can find the effective pathway targets for drug development and application.Therefore,by analyzing the differentially expressed mRNA in papillary renal cell carcinoma.we identified the effective differential genes and found an effective pathway for their expression,which was beneficial to the study of subsequent targeted drugs.ObjectiveThe present study aims to investigate the gene expression changes in papillary renal cell carcinoma(pRCC)and screen several genes and associated pathways of papillary renal cell carcinoma progression.MethodsThe papillary renal cell carcinoma RNA sequencing(RNA-seq)data set and clinical data were downloaded from TCGA(The Cancer Genome Atlas).We identified the differentially expressed mRNAs between cancer and normal tissues and performed annotation of differentially expressed mRNAs to figure out the functions and pathways they were enriched in.Then,we constructed a risk score that relied on the 5-mRNA.The optimal value for the patients' classification risk level was identified by ROC analysis.The relationship between mRNA expression and prognosis of papillary renal cell carcinoma was evaluated by univariate Cox regression model.The 5-mRNA based risk score was validated in both complete set and testing set.Results1.The five mRNAs(CCNB2,IGF2BP3,KIF18A,PTTG1 and BUB 1)were significantly associated with the survival prognosis of patients in papillary renal cell carcinoma.2.The pathway regulation involved in the five mRNAs of CCNB2,IGF2BP3,KIF18A,PTTG1 and BUB1 can provide new targets for the development of targeted drugs.3.The patients with high expression of CCNB2,IGF2BP3,KIF18A and PTTG1 genes had poor prognosis.4.The patients with high expression of BUB 1 had better prognosis.ConclusionThe five differentially expressed mRNAs(CCNB2,IGF2BP3,KIF18A,PTTG1,and BUB1)can be used as a potential marker for prognosis in patients with papillary renal cell carcinoma and provide a new targeted drug study for their pathways.