| Objective:To explore the value of combination application of serum chemokines CXCL8 and CCL20 in predicting liver metastasis of colorectal cancer,and provide new methods and ideas for clinical diagnosis of colorectal liver metastasis.Methods:A total of 80 patients with colorectal cancer were diagnosed by pathology at the Third Affiliated Hospital of Kunming Medical University from December 2017 to February 2018,including 40 patients without metastasis and 40 patients with liver metastasis,called the experimental group one,the experimental group two.At the same time,40 healthy volunteers who were examined in our hospital during the same period were selected as the control group.The clinical basic information was collected,5 ml of venous blood was taken,and serum was separated by centrifugation to detect the expression of CXCL8 and CCL20.Using SPSS 23.0 software,Analysis of Variance(ANOVA)was used to compare the difference of CXCL8 and CCL20 expression between the three groups,and the least significant difference(LSD)was used for post hoc comparison.Liver metastasis was the gold standard,and Receiver Operating Characteristic(ROC)was used to evaluate the value of combined detection of CXCL8 and CCL20 in predicting liver metastasis of colorectal cancer,including sensitivity,specificity,and area under the ROC curve(Area Under Curves,AUC).Chi-square test was used to analyze the correlation between the experimental group one and the experimental group two clinical information,as well as the correlation between CXCL8 and CCL20 expression and clinical indicators.Results:1.The serum CXCL8 values of the experimental group one,the experimental group two and the control group were 38.76±4.785ng/ml,97.88±8.286 ng/ml and 14.55±0.4617 ng/ml,respectively;the serum CCL20 values of the experimental group one,the experimental group two and the control group were 19.18±1.205ng/ml,32.68±3.688 ng/ml and 15.13 ± 0.4219 ng/ml,respectively.The differences of CXCL8 and CCL20 between the three groups were statistically significant.Among them,the differences between CXCL8 and CCL20 in the experimental group one and the control group were statistically significant(P<0.0001,P=0.0022);the differences between CXCL8 and CCL20 in the experimental group one and the control group were statistically significant(P<0.0001).P<0.0001);the differences between CXCL8 and CCL20 in the experimental group one and the experimental group two was statistically significant(P<0.0001,P=0.0008)2.The high expression rates of CXCL8 in the experimental group one and the experimental group two were 20%(8/40)and 80%(32/40),respectively.The high expression rate of CCL20 in the experimental group one and the experimental group two was 32.5%(13/40)and 67.5%(27/40);the expression of CXCL8 and CCL20 in the experimental group one and the experimental group two were statistically significant(?2=28,8,P<0.001;x2=9.8,P=0.002).The high expression rates of CCL20 and CXCL8 in the experimental group one and the experimental group two were 2.5%(1/40)and 57.5%(23/40),respectively,and the difference was statistically significant(x2=28.81,P<0.001);colorectal there was a significant correlation between the expression of CXCL8 and CCL20 in the serum of patients with cancer(r=0.316,P=0.004);The liver metastasis and lymph node metastasis of colorectal cancer(P=0.003),CXCL8 concentration(P<0.001),CCL20 the concentration(P=0.002)was correlated;the high expression of CXCL8 was associated with tumor site(P=0.007),depth of invasion(P<0.001),tumor differentiation(P=0.021),liver metastasis(P<0.001);The high expression of CCL20 and tumor infiltration depth(P=0.025)and liver metastasis(P=0.002)were associated;CXCL8 and CCL20 were both highly expressed and tumor diameter(P<0.001),tumor infiltration depth(P=0.01),lymph node metastasis(P=0.04)and Liver metastasis(P<0.001)was associated with no significant association with other basic clinical information3.The sensitivity,specificity and AUC value of CXCL8 for colorectal liver metastasis were 80%,80%,and 0.859,respectively.The sensitivity,specificity and AUC value of CCL20 for colorectal liver metastasis were 67.5%,67.5%,0.704,respectively.The sensitivity,specificity,and AUC values of combining with CXCL8 and CCL20 for colorectal liver metastasis were 57.5%,97.5%,and 0.884,respectively.Combined with CXCL8 and CCL20,the AUC value of colorectal liver metastasis was higher than that of CXCL8 and CCL20 alone.The difference was statistically significant(P=0.2587,P=0.0021),the specificity of combined CXCL8 and CCL20 for predicting colorectal liver metastasis is higher than that of CXCL8 and CCL20 alone.But the sensitivity of combining with CXCL8 and CCL20 to predict colorectal liver metastasis did not improve,and the CXCL8 alone prediction and the combined with CXCL8 and CCL20 prediction is no difference in AUC values,while CXCL8 alone predicted AUC values significantly higher than CCL20 and the difference was statistically significant.Conclusion(s):1.Serum CXCL8 and CCL20 were higher in colorectal cancer patients than in healthy volunteers.2.Serum CXCL8 and CCL20 in colorectal cancer liver metastasis patients were also higher than those in colorectal cancer without metastasis.3.There is a positive correlation between the expression of CXCL8 and CCL20 in serum of patients with colorectal cancer.4.Serum CXCL8 and CCL20 are helpful in predicting liver metastasis of colorectal cancer.The value of CXCL8 predicts colorectal liver metastasis is higher than CCL20;The value of combining with CXCL8 and CCL20 predicts colorectal liver metastasis is not better than the value of CXCL8 alone predicts colorectal cancer livermetastasis.But the combination of CXCL8 and CCL20 predicts the extremely high specificity and AUC value of liver metastasis of colorectal cancer,suggesting that the combined detection of CXCL8 and CCL20 has a tendency to be the standard for liver metastasis of colorectal cancer.5.Combined detection serum of CXCL8 and CCL20 may be helpful in clinical evaluation of liver metastases in patients with colorectal cancer. |
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