| Aim:To explore the effect of oxidative stress induced by HIF-1 ? on fetal growth and development,to analyze the correlation between serum HIF-1 ? and urine 8-OHd G,and to evaluate the value of HIF-1 ? and 8-OHd G in predicting fetal growth abnormality.Methods:From December 2017 to February 2019,60 pregnant women were selected from Qingdao municipal hospital for standard birth examination and delivery.The pregnant women with 32-36 weeks' diagnosis of fetal growth restriction(FGR)or slow growth were taken as the study group,and 30 healthy pregnant women with normal fetal development were taken as the control group,and were followed up to delivery.Collect relevant information: 1)basic information: age,gestational times,birth times,gestational weeks of delivery,weight gain during pregnancy,birth weight of newborn,placenta weight;2)take pre diagnosis and delivery as the time to take blood samples and urine samples,and take placenta tissue during delivery.The levels of HIF-1 ? in maternal plasma and 8-OHd G in urine were detected by ELISA.Western blot was used to detect the expression of HIF-1 ? protein in placenta.And the data was analysed using the statistical software SPSS 22.0.Results:(1)Basic document: there was no significant difference between the study group and the control group in terms of age,gestational times,birth times and weight gain during pregnancy(P > 0.05).The birth weight,placental weight and gestational weeks of delivery in the study group were significantly lower than those in the control group,and the difference was statistically significant(P<0.05).(2)Analysis of the level of serum HIF-1 ?: The level of serum HIF-1 ? in the study group and the control group decreased with the increase of gestational age,and the difference was not statistically significant;the level of serum HIF-1 ? in the study group was significantly higher than that in the control group(5337.79 ± 1689.02 pg / ml >4083.29 ± 1617.29 pg / ml,P < 0.05),and the difference was statistically significant.(3)Analysis of urine 8-OHd G level: the urine 8-OHd G level in the control group slightly increased with the gestational age,13.64 ± 1.52ng/ml < 13.69 ± 1.63ng/ml,the difference was not statistically significant;the urine 8-OHd G level in the study group gradually decreased with the increase of gestational age(diagnosis period: 14.86 ±1.62ng/ml,delivery period: 13.83 ± 1.62 ng / ml),the difference was not significant.The level of 8-OHd G in urine of the study group was higher than that of the control group(14.86 ± 1.62 ng / ml > 13.64 ± 1.52,P < 0.05),the difference was statistically significant.(4)There was no correlation between plasma HIF-1 ? and urine 8-OHd G levels in FGR and normal pregnant women.(5)The expression level of HIF-1 ? protein in placenta of study group was significantly higher than that of control group(1.19 ± 0.35 > 0.81 ± 0.20),P < 0.05,the difference was statistically significant.(6)ROC curve analysis: the sensitivity of serum HIF-1 ?,urine 8-OHd G and their combined indexes to the prediction of fetal growth and development is 75%,85% and70%,the specificity is 73%,78% and 90%,AUC is 0.739,0.783 and 0.833,respectively.The combined indexes of the two indexes show the greatest specificity and AUC.Conclusion:1.The oxidative stress injury induced by HIF-1 ? may affect the growth and development of fetus,and thus participate in the occurrence of FGR.2.As a marker of oxidative stress injury,urinary 8-OHd G has a certain predictive value for fetal growth and development abnormality;the local oxidative stress injury of placenta induced by HIF-1 ? may be one of the reasons for fetal growth and development abnormality.3.In view of the influence of oxidative stress on fetal development,urinary 8-OHd G may be used as a predictor of fetal growth and development. |
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