Expression Of ASPP Family In Cutaneous Squamous Cell Carcinoma And Its Clinical Significance

BackgroundCutaneous Squamous Cell Carcinoma(Cutaneous Squamous Cell Carcinoma,CSCC)for short,is one of the most common malignant tumors in dermatology.Its incidence is second only to basal cell carcinoma and originates from the skin epidermis and its appendages(hair follicle funnel,sebaceous ducts,end sweat ducts)non-melanoma skin malignancies of keratinocytes.The p53 gene is the gene most closely related to tumor pathogenesis found to date.This gene is a tumor suppressor gene and its encoded product is the P53 protein,which can promote DNA repair,block cell cycle,inhibit cell proliferation,and promote cell decay,Death,inhibition of angiogenesis and many other effects.The normal functioning of P53 depends on the synergistic effects of various regulatory factors,including the P53 apoptosis stimulating proteins gene family(ASPP).The family is composed of ASPP1(apoptosis stimulating proteins 1 of P53),ASPP2(apoptosis stimulating proteins 2of P53),and iASPP(inhibitory member of the ASPP family).Abnormal expression of ASPP family can lead to the occurrence and development of many tumors.At present,there are no reports on the expression of ASPP gene family in CSCC tissues abroad,and there are only relevant reports on the expression of iASPP in CSCC in China.PurposeThe expression of ASPP1,ASPP2,and iASPP in CSCC are investigated by immunohistochemical methods,that is,immunohistochemistry,and its various clinical pathological characteristics,such as gender,age,location,tumor size andtumor invasion,and the role of these three substances in the pathogenesis of CSCC and the significance of clinical diagnosis are explored.MethodsThe expressions of ASPP1,ASPP2 and iASPP in 40 cases of CSCC tissue and 15 cases of normal skin tissue are detected by immunohistochemistry.The expression results of immunohistochemistry are observed under a microscope.We use SPSS 22.0statistical software to processe and analyze the specific data of this experiment,and use ?2 test to describe the expression of ASPP1,ASPP2,and iASPP and analyze the relationships of various clinicopathological characteristics of patients with CSCC,such as gender,age,location,tumor size and tumor invasion.Spearman's test is used to test the correlation between ASPP1,ASPP2 and iASPP.The test level is ? = 0.05,and P <0.05 is considered statistically significant.Results1.ASPP1 protein expression: mainly expressed in the cytoplasm.The expression rate in CSCC is 30.00%,and the expression rate in normal skin tissue is 33.33%.Compared with the two,P = 1.000(P> 0.05),which is not statistically significant.2.ASPP2 protein expression: mainly expressed in the cytoplasm.The expression rate in CSCC is 15.00%,and the expression rate in normal skin tissue is 40.00%.Compared with the two,P = 0.103(P> 0.05),which is not statistically significant.3.iASPP protein expression: mainly expressed in the cytoplasm,and highly expressed in the cancer nest.The expression rate in CSCC is 40.00%,and the expression rate in normal skin tissue is 6.67%.Compared with the two,P=0.04(P<0.05),which is statistically significant.4.The expression levels of ASPP1 and ASPP2 in CSCC tissues are not related to the sex,age,location,tumor size and invasiveness of tumor patients,and there are no significant difference(P> 0.05).5.The expression level of iASPP in CSCC tissues is not related to the sex,age,location,tumor size in tumor patients,and there is no significant difference(P> 0.05).But the expression level of iASPP in aggressive CSCC is significantly higher thanthat in situ CSCC,and there is a significant difference(P <0.05).6.Spearman correlation analysis shows that the expression levels of ASPP1,ASPP2 and iASPP in CSCC tissues are not correlated with each other(P>0.05).7.Spearman correlation analysis shows that the expression levels of ASPP1,ASPP2 and iASPP in normal skin tissues are not related to each other(P> 0.05).Conclusions1.iASPP is positively expressed in CSCC tissues and highly expressed in cancer nests.2.The expression levels of ASPP1 and ASPP2 in CSCC tissues are not related to the sex,age,location,tumor size and invasiveness of tumor patients.3.The expression level of iASPP in CSCC tissues is not related to the sex,age,location,tumor size in tumor patients,but it is related to its aggressiveness.4.The expression levels of ASPP1,ASPP2 and iASPP in CSCC tissues are not related to each other.