Effect Of UNC5B-AS1 On Migration And Invasion Of Gastric Cancer Cells

Objective: To investigate the effect of lncrna unc5b-as1 on the migration and invasion of gastric cancer cells.Methods: 1.The original data of human gastric cancer gene sequencing were downloaded from TCGA(the Cancer Genome Atlas)and geo(Gene Expression Omnibus)databases,and the mRNAs and lncrnas with significant expression differences were screened by de SEQ software package(logFC ? 1 or logFC?-1,P < 0.01 was considered as significant differences).2.Using the original data of human gastric cancer samples in gepia database,we predicted the correlation between the expression of lncrna unc5b-as1 and the clinical characteristics such as total survival time,relapse free survival time.3.The expression of unc5b-as1 was detected by real-time fluorescence quantitative reverse transcription PCR(qRT-PCR).4.CCK-8 assay was used to detect the effects of knockdown and overexpression of unc5b-as1 on the viability of gastric cancer cells.5.The effect of knockdown and overexpression of unc5b-as1 on the migration and invasion of gastric cancer cells was detected by scratch and invasion experiments.6.MiRBase,mirtarbase and mirdb websites were used to predict the Cerna mechanism of UNC5B-AS1-hsa-miRNA-4632-5p-KMT2 A.7.Rpiseq database is used to predict whether or not unc5b-as1 and kmt2 a are directly combined and where they are combined.Results: 1.The results of TCGA and GEO sequencing showed that the expression of UNC5B-AS1 in human gastric cancer was significantly lower than that in adjacent adjacent adjacent tissues.2.The expression of UNC5B-AS1 had no significant effect on the overall survival(OS)of gastric cancer patients.Further clinical correlation analysis of UNC5B-AS1 showed that patients with high expression of UNC5B-AS1 had poor disease free survival(DFS),and higher expression of unc5b-as1 in stage ?-? compared with stage?.3.The results showed that the expression of unc5b-as1 was significantly lower than that of paracancerous tissues.4.Knockdown of UNC5B-AS1 in IM95 and MKN-45 cells enhanced the migration and invasive abilities of gastric cancer cells,overexpression of UNC5B-AS1 inhibited the invasive ability of gastric cancer cells.5.Through the miRBase website,we further predicted the miRNA points of unc5b-as1.The results showed that UNC5B-AS1 might be used as the sponge of hsa-miRNA-4632-5p.Therefore,we further used mirtarbase and mirdb website to further predict the mRNA that may bind to hsa-miRNA-4632-5p,and the results show that a total of 21 mRNA may bind to it.Combined with KEGG pathway analysis,we found that kmt2 a and kmt2 d were significantly enriched in lysine degradation pathway.In the GSE54129 data of 111 cases of gastric cancer,we found that there was a significant negative correlation between the expression of UNC5B-AS1 and KMT2 A,but there was no significant correlation between the expression of UNC5B-AS1 and kmt2 d.Finally,we use RPISeq database to further predict the direct combination of UNC5B-AS1 and kmt2 a.The results show that the 3 'and 5' end sequences of unc5b-as1 may be significantly combined with kmt2 a.Conclusions: Inhibition of migration and invasion of gastric cancer cells by unc5b-as1 down regulating kmt2 a by binding with kmt2 a protein.