| Background and Objective:Lung cancer is one of the most common malignant tumors in China and even in the world,which seriously affects the safety of human life,and non-small cell lung cancer accounts for 85%of all kinds of lung cancer.At present,the research on the screening and treatment of non-small cell lung cancer is increasing.PET/CT,as the most popular molecular imaging advanced equipment,is also used in the detection of more non-small cell lung cancer.Compared with the traditional imaging examination,PET/CT can not only provide the anatomy of NSCLC,but also provide the tumor metabolism information that the traditional imaging examination cannot provide.18F-FDG PET/CT can detect most tumor lesions in early stage and carry out accurate staging to guide further treatment plan.However,clinical practice shows that the uptake of 18F-FDG in NSCLC is quite different,even when the clinical factors such as pathological type and stage are the same.In order to find out the reason of the difference of 18F-FDG uptake in NSCLC,there are studies abroad from the point of view of GLUT1 gene,also SLC2A1 gene polymorphism.At present,there are no articles about SLC2A1 gene polymorphism and 18F-FDG uptake in NSCLC in China.So in this study,XbaI G>T(rs841853)and-2841A>tT(rs710218)were selected as two sites with high mutation rate in Asian population to explore the correlation between SLC2A1 gene polymorphism and 18F-FDG uptake in non-small cell lung cancer.,and to provide laboratory basis for clinical individualized diagnosis and treatment of non-small cell lung cancer.Materials and methodsThe First Affiliated Hospital of Zhengzhou University was selected as 18F-FDG in our hospital from October 2018 to March 2019 112 cases of non-small cell lung cancer diagnosed by PET/CT were confirmed by pathology.After obtaining informed consent,the peripheral venous blood of 2ml patients was extracted.The whole blood DNA was extracted and PCR was carried out.The amplification results were detected by 1.5%-2%agarose gel electrophoresis,and the final sequencing was carried out by Beijing three Bo Yuan Zhi Biotechnology Co.,Ltd.The goodness of fit was tested by?2test,the measurement data were described by X±s,the comparison between the two groups or the comparison between the two groups was analyzed by t-test and one-way ANOVA,and the correlation between the two groups was analyzed by one-way regression analysis,P<0.05.Results:There was no significant difference between age,18F-FDG pre PET/CT blood glucose concentration and 18F-FDG injection dose and SUVmax in non-small cell lung cancer patients(P>0.05);regression analysis showed that the length of lesions in non-small cell lung cancer patients was positively correlated with 18F-FDG concentration,R2=0.362,the difference was statistically significant;The results of t-test showed that there was no significant difference between gender and small cell lung cancer patients in SUVmax,but higher in squamous cell carcinoma than in adenocarcinoma.The difference was statistically significant(P<0.05).There was no significant difference in SUVmax among the three genotypes NSCLC of Xbal G>T locus by one-way ANOVA.There was significant difference in SUVmax between GT and TT genotypes combined to form dominant model group and wild type non-small cell lung cancer(P<0.05).The concentration of 18F-FDG was higher in dominant model non-small cell lung cancer;There was no significant difference in SUVmax between recessive model group and mutant homozygous group.There was no significant difference in SUVmax of three gene type NSCLC by One way ANOVA or T test among the-2841 A>T loci(P>0.05).There was no significant difference in SUVmax of three gene type adenocarcinoma of lung by One way ANOVA or T test among the Xbal G>T loci(P>0.05),also GT genotypes and GG gene type squamous cell carcinoma of lung by T test(P>0.05).There was no significant difference in SUVmax of three gene type adenocarcinoma of lung by One way ANOVA or T test among the-2841 A>T loci(P>0.05),also AT and TT genotypes combined to form dominant model group and AA gene type squamous cell carcinoma of lung by T test(P>0.05).Conclusion1.SLC2A1 gene polymorphism can affect the uptake of 18F-FDG in NSCLC;Xbal G>T gene mutation in NSCLC patients may uptake more 18F-FDG;-2841 A>T gene mutation does not affect the uptake of 18F-FDG in NSCLC patients.2.The uptake of 18F-FDG in NSCLC can be affected by the length of lesions,pathological types and clinical stages.3.Sex,age,blood glucose before PET/CT and 18F-FDG injection dose witnin the standard range were not related to the uptake of 18F-FDG by non-small cells. |
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