| Objective To investigate the effect of preservation of ligament stump on tendon healing in anterior cruciate ligament reconstruction.Methods Forty New Zealand rabbits were randomly divided into two groups: ACL reconstruction group without remnant preservation(group A)and ACL reconstruction group(group B)with remnant preservation,there were 20 rabbits in each group.The Achilles tendon was selected as the graft,and the bilateral ACL of all the rabbits were cut off.In group A,the ACL stump was completely removed.In group B,the tibia stump was cut off from the femoral stop,and the tibia stump was retained.According to the position of the ACL of the normal rabbits,the tibia and the femoral canal were selected for reconstruction.The percentage of positive cells of HIF1-? and VEGF were detected and the signal intensity of tendon and the width of bone tunnel were observed by MRI.Results The percentage of HIF-1? and VEGF positive cells in group B was significantly higher than that in group A at 6 weeks after operation.At 12 weeks after operation,The percentage of HIF-1? and VEGF positive cells in the two groups decreased 12 weeks after operation compared with that in the same 6 weeks group(P < 0.05),and there was no significant difference between the two groups(P > 0.05).Imaging results: 6 weeks after operation,the expansion of bone tunnel in group B was significantly lower than that in group A,and the signal intensity of tendon graft was lower than that in group A(P < 0.05).After 12 weeks,the expansion degree of bone tunnel and the signal intensity of tendon were decreased in both groups(in the same group P < 0.05,between the two groups P > 0.05.)Conclusion :In this experiment,in the early stage of ligament reconstruction,the ACL reconstruction with remnant preservation is superior to the ACL reconstruction without remnant preservationin terms of graft revascularization and reduction of bone tunnel expansion,showing some biological advantages. |
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