Establishment Of Reference Interval Of 25-hydroxy-vitamin D In Adult Serum In Urumqi

Objective: This study is to establish and verify the reference interval of 25(OH)D for healthy adults in Urumqi by direct method and indirect method,to evaluate the advantages and disadvantages of the two methods and to compare the difference between the two methods.Methods: According to the inclusion and exclusion criteria,710 subjects' data were selected as the study object by direct method,and 25(OH)D data of 9745 physical examinees in hospital laboratory information management system(LIS)were selected as the study object by indirect method.Using Roche electrochemiluminescence instrument,under the condition of ensuring the quality of the instrument is under control,25(OH)D of the selected samples is detected.According to the guidelines of the United States Committee for clinical Standardization(CLSI)EP28-A3 c,two methods are used to establish the reference interval.Results: The total reference interval established by direct method was 6.32-32.66 ng/mL for men,6.58-34.18 ng/mL for women,6.21-29.75 ng/mL for men18-35 years old,5.32-32.82 ng/mL for men aged 36-60,7.74-38.83 ng/mL for men aged 36-60,6.51-31.28 ng/mL for women aged 36-60,6.22-27.40 ng/mL for women aged > 60,Han reference interval: 6.33-33.36 ng/mL,Uygur reference interval: 6.24-28.90 ng/mL,spring reference interval: 5.22-33.68 ng/mL,summer reference interval: 6.73-32.49 ng/mL,autumn reference interval: 7.14-39.07 ng/mL,winter reference interval: 6.01-24.98 ng/mL.The overall reference interval of indirect method is 7.08-35.07ng/mL,the reference interval of men is 8.37-36.76 ng/mL,the reference interval of women is 6.37-31.59 ng/mL,the reference interval of men18-35 years old is 8.65-36.11 ng/mL the reference interval of women18-35 years old is 5.87-25.73 ng/mL,the reference interval of men 36-60 years old is 8.30-36.31 ng/mL,the reference interval of women 36-60 years old is 6.52-31.59 ng/mL,reference interval for men over 60 years old: 7.79-40.30 ng/mL,reference interval for women over 60 years old: 6.89-36.33 ng/mL,reference interval for spring: 6.60-32.85 ng/mL,reference interval for summer: 7.87-34.48 ng/mL,reference interval for autumn: 7.30-35.73 ng/mL,reference interval for winter: 6.10-34.54 ng/mL.Conclusion: There is no statistical difference between the results of direct method and indirect method in establishing the reference interval of 25(OH)D in this region,which can be applied to this region.