Establishing Reference Intervals Of Pepsinogen By Analysis Of Laboratory-stored Data

Objective:The reference interval of pepsinogen was established through the data in the laboratory information system,and the feasibility of the reference interval of pepsinogen established in the laboratory in this study was verified by using the EP28—A3document proposed by the American clinical standardization committee as the scientific criteria.To explore the effects of gender,age and nationality on pepsinogen(PG).If there is any effect,the reference interval of serum PGIn our laboratory should be established in groups to provide reference for the early screening of gastric diseases.Methods:The basic personal information and test results of PG project were collected from the information management system of the clinical examination laboratory of the third clinical medical college of xinjiang medical university from November 2016 to November 2018,including physical examination patients over the age of 18 with apparent health and previous physical health and outpatient patients.According to the inclusion and exclusion criteria,the test results of more than 1962 patients were collected through data collection and collation.The detection results of all PG projects were detected by i2000 automatic particle chemiluminescence immunoanalyzer produced by abbott.Statistical software SPSS19.0 was used and appropriate statistical methods were used for data statistical processing to calculate the 95% reference interval of all test results.Then,groups were divided according to gender,age and ethnic group,to explore the influence of gender,age and ethnic group on PG,and to establish a reference interval for grouping.The feasibility of the reference interval of pepsinogen established by the laboratory in this study was verified by the EP28—A3 document,and was compared with the reference interval used by the laboratory at present.The results were statistically significant with P < 0.05.Results:The reference intervals established in this laboratory were PGI 28.58-77.74 ng/ml,PGII?20.5 ng/ml,and PGR?3.1;the original intervals were PGI 70-200ng/ml,PGII?20ng/ml,and PGR?3.The difference between the new reference interval and the original reference interval of PGI was statistically significant(P < 0.05).The difference of PGI and PGR detection results between different genders was statistically significant(P < 0.05),while the difference of PGII detection results between different genders was not statistically significant(P > 0.05).The established reference intervals were: male PGI:32.06-84.78ng/ml,female PGI: 27.03-75.55 ng/ml.PGR? 3.63 for males and 2.79 for females;PGII acuities were 20.5 ng/ml.The differences of PGR detection results among different RACES were statistically significant(P < 0.05),while the differences of PGI and PGII detection results among different RACES were not statistically significant(P > 0.05).The established reference interval is: PGI 39.65-80.61ng/ml;PGII acuities were 20.5ng/ml;PGR ? 3.3 for han nationality and 3.04 for non-han nationality.There was no significant correlation between the measured results of PG and patients of different ages(P< 0.05).However,the correlation coefficient r < 0.3 is poor.Conclusion:In this study,we established the RIs of PGIn our laboratory,which has certain guiding value for the future clinical testing center laboratory to establish reference intervals of different laboratory indicators according to its own conditions.