Effect Of Glucagon-like Peptide-1(GLP-1)on MafA-deficient Mice

Objective:To investigate effect of glucagon-like peptide-1?GLP-1?on MafA-deficient mice.Materials and Methods:Ten 6-week-old male MafA knockout mice were selected and randomly divided into 2 groups of 5 mice each.Ten ICR wild-type mice of the same age were randomly divided into two groups of five.GLP-1 or physiological saline?50?g/kg body weight?was given to the four groups,one kind for each group twice a day for 4weeks.The grouping is as follows:MafA knockout mice:MafA^-/-GLP-1 group,MafA^-/-saline group;ICR wild-type mice:wild GLP-1 group,wild saline group.Record of blood glucose level and body weight were kept in each group;and immunohistochemical sections were used to count and analyze the number of islet?cells and?cells in each experimental group,the?cells/?cells ratio was calculated,and the Real-time fluorescence quantitative PCR flurogenic quantitative polymerase chain Reaction?FQ-PCR?was applied to analyze the changes of related transcription factors Pdx-1,FoxO1,and insulin I and so on.Results:In the absence of MafA gene,compared with MafA^-/-saline group,the blood glucose level of MafA^-/-GLP-1 group was significantly improved,and the difference was statistically significant?p<0.01?;indicators notes obvious decline in food intake and loss of weight?p<0.05?;while for the pancreas sections,?/?cells ratio is increased?2.25±0.22/2.67±0.37?,which is considered statistically significant with?p<0.05?.By real-time fluorescence quantitative PCR,it was found that PDX-1mRNA in MafA^-/-GLP-1 group was significantly increased?p<0.01?;FoxO1mRNA level decreased significantly?p<0.05?Conclusions:1.GLP-1 can significantly improve blood glucose levels in MafA knockout mice.2.GLP-1 can reduce food intake and body weight of MafA knockout mice.3.GLP-1 is possible to increase the regeneration of?cells by inducing PDX-1and then promoting?cells to islet?cellstransdifferentiation,or through reversion of dedifferentiation of islet?cells by inhibiting FoxO1.