| Objective: To observe the effect of Platelet rich fibrin(PRF)on the healing of diabetic wounds,and to explore its effect on the phenotypic transformation of macrophage and wound vascularization in diabetic wounds during the process of diabetic wounds,and to explore the mechanism of PRF promoting the healing of diabetic wounds.Methods: A total of 45 rats were selected to establish a diabetic model.Wounds were made on the bilateral symmetry of the dorsal spine in diabetic rats.The wounds on both sides were divided into PRF group and control group.The wounds of the PRF group were covered with 15 ml of blood-extracted PRF gel and covered with petrolatum oil sands.The control wounds were directly covered with petroleum jelly.On the 3d,7d and14 d after wound treatment,the wound healing was observed,the wound healing rate was counted,and some rat wound tissues were fixed with formaldehyde solution,paraffin embedded and serial sections,and finally immunized with CD34,CD86 and CD163.Histochemical staining was performed to calculate the microvessel density at 7 days after wound treatment and the relative density of M1 macrophages and M2 macrophages at each time point.Results: 28 of the 45 rats were successfully made into a diabetic rat model with a success rate of 62.2%.The wound healing rate of PRF group was significantly higher than that of the control group at 3d,7d and 14 d after wound treatment,and the difference was statistically significant(P<0.05).The results of CD34 immunohistochemistry showed that the microvessel density in the PRF group was significantly higher than that in the control group at 7 days after operation(P<0.05).The results of immunohistochemistry of CD86 and CD163 showed that M1 macrophages in PRF group were significantly less than those in control group at 3,7 and 14 days after operation,and M2 macrophages were significantly more than control group(P<0.05).Conclusion: PRF may promote the healing of wounds by promoting microvascular angiogenesis and regulating macrophage phenotypic changes,promoting vascularization and inhibiting local inflammatory reaction of wounds,and providing theoretical support for the clinical application of PRF in diabetic wounds. |
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