| Objective:When HIV-1 enters the human body,in addition to infecting its main target cell CD4+T lymphocytes,it can also infect mononuclear and macrophages,and can invade the central nervous system through the blood-brain barrier,resulting in a series of pathological changes and neurological impairment,it also known as "HIV-Associated Neurocognitive Disorders"(HAND).The penetration of HIV-1 though the blood-brain barrier is considered to be the key to the occurrence of HAND,and its mechanism is still unclear.lt is currently believed that monocytes carrying HIV-1 migrate to the brain through the blood-brain barrier and may play an important role in the development of HAND.TheG protein-coupled receptor CD181(CXCR1)is a receptor for the chemokine IL-8(CXCL8)and is capable of specifically binding to IL-8.Some scholars have found that IL-8 can not only recruit and activate neutrophils,but also regulate the inflammatory reaction of monocytes,basophils,B cells and T cells,increase the expression of viral genes,and help monocytes migrate through the blood-brain barrier.Our previous study found that the expression of CD181 on monocytes in patients with HAND is significantly higher than that in non-HAND.And the monocyte subsets which CD181 mainly exists in,ie CD14+CD16+monocytes,have more advantages in trans-membrane migration and integration of viral DNA.The content of HIV-1 DNA in cerebrospinal fluid of HAND patients is also increased.However,does HIV-1 infection affect the expression of CD181 in monocytes?Do CD 181+monocytes carry HIV?No report has been reported yet.In order to investigate the relationship between monocytes and HAND,we intend to study the expression of CD181 and the content of HIV DNA in monocytes of HIV-infected patients,so as to provide a preliminary basis for further understanding the role of monocytes in the pathogenesis of HAND in HIV-infected patientsMethods:1.Research subjects.According to the national HIV/AIDS diagnostic criteria of China,from Aug 2018 to Jan 2019,16 patients in the Red Ribbon Clinic of the First Affiliated Hospital of China Medical University was selected who did not receive antiviral therapy and confirmed positive HIV-1 infection,and 20 healthy people in the same periodas the control group.2.Extract peripheral blood mononuclear cells(PBMC)3.After sorting mononuclear cells by Easy SepTM negatively separating kit,the CD181 positive and negative monocytes were sorted by FASCAriall flow cytometry,andsimultaneously the expression level of CD181 on monocytes and lymphocytes was detected.4.Extract the DNA of monocytes according to the Qiagen DNA Extraction Kit instructions.5.Measure the average copy number of HIV-1 DNA in each monocyte by dd-PCR.6.Statistical analysis.The experimental data were analyzed and graphed by SPSS22.0 and GraphPad Prism7 software.The difference between groups was analyzed by t test.The correlation analysis was performed by Pearson coefficient test.P<0.05 was considered statistically significantResults:1.C1.Compared with the healthy control group,the expression of CD181 on the surface of monocytes in HIV-1 infected patients increased significantly2.Compared with the healthy control group,the expression of CD181 on the lymphocyte surface of HIV-1 infected patients increased significantly3.The expression of CD181 on monocytes of HIV-1 infected patients was significantly higher than that on lymphocytes4.The HIV-1 DNA content of CD181+monocytes was significantly higher than that of CD 181-monocytes.ConclusionsrCD 181 expression is significantly increased in monocytes of HIV-1 infected individuals,and CD 181+monocytes carry more HIV-1 DNA than CD 181-monocytes. |
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