Design And Research Of An In Virtro Model For Thermal Treatment Based On 3D Cell Culture

Thermal therapy is a minimally invasive and dose free therapy.Combination of thermal therapy and chemotherapy or immunotherapy can enhance the curative effect for highly invasive tumors.The development of this kind of therapy requires a series of evaluation in vitro before clinical application.However,the present cell thermal experiment are mainly conducted through heating the attached or suspended cells,which can't simulate the complex micro-environment in vivo and may finally influence the accuracy of the experiment.In order to overcome these limitation and build up an in vitro model for the thermal therapy,the present study tried to design a petri dish which is able to heating the cells cultured both in 2-D and 3-D through laserinduced etching of conductive glass(ITO),feedback controlling of temperature and 3-dimensional cell culture in alginate.And at the same time,we can constantly monitor the condition of cells through the microscope.According to the experiment,the petri have the superiority of biocompability,visuality,portability,accuracy and stability,while the 3D cell culture is used to simulate the micro-environment of the tumor in vivo which is a effective to heat and culture the cells.In the end,this study use the above-mentioned heating system to heat the Mcf-7 tumor cells to test whether the system is usable.During the experiment,we successfully monitored the change of cell shape which is caused by the degradation of the cytoskeleton.Also,after heating,we tracked the change of cellular proliferation speed and observed the inhibition of the proliferation.The experiment proved that the cell heating and culturing system is an accurate instrument for cellular experiment.