| Background:Previous studies demonstrated that the survival time of chondrocytes is prolonged in three-dimensional culture system of multifactorial sodium alginate; extracellular matrix has a strong secretion.Objective:To explore the effect of biological stability of long-term amplification culture chondrocytes on three-dimensional culture system of multifactorial sodium alginate.Methods:Chondrocytes were implanted on three-dimensional culture system which comprises sodium alginate, hyaluronic acid, chitosan, fibronectin, and basic fibroblast growth factor (bFGF), and the plane culture as controls. The morphological changes, differences of cell growth curves, and the contents of glycosaminoglycan (GAG) of cells supernatant fluid of chondrocytes were regularly observed in two different culture systems.Results:Morphous of chondrocytes on three-dimensional culture system of multifactorial sodium alginate grew well and flourishingly, and formed the globular group of cells, cell proliferation was active. Proliferation of chondrocytes on the culture system of multifactorial sodium alginate was slightly higher than that implanted on plane cultured 2 days. Proliferation of chondrocytes was significantly higher cultured 4 days on the culture system of multifactorial sodium alginate. Chondrocytes were keep stable proliferation at 6-14 days culture; while the proliferation of chondrocytes cultured on plane was gradually decrease. The contents of GAG of cells supernatant fluid of the three-dimensional culture system were significantly higher than monolayer culture system; chondrocytes express typeâ…¡collagen and GAG on the culture system of multifactorial sodium alginate significantly higher than monolayer culture system.Conclusion:Chondrocytes on three-dimensional culture system of multifactorial sodium alginate can long-term and rapid amplification culture and keep their biological stability. |
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