The Effect On PH?Na⁺?K⁺for IBS Washing Blood And Intracellular Ca²⁺ And PS Expression On Membrane For IBS Red Blood Cells Of Multiple Electrolytes Injection

Objective:To observe the effect on pH?Na⁺?K⁺for IBS?Intraoperative Blood Salvage?washing blood and intracellular Ca²⁺and PS?phosphatidylserine?expression on membrane for IBS red blood cells of multiple electrolytes injectionMethods:Twenty patients who underwent IBS vertebral surgery,were randomly divided into normal saline group?group N,n=10?and multiple electrolytes injection group?group M,n=10?.The blood was washed by normal saline in group N and multiple electrolytes injection in group M during IBS.The arterial blood was taken for detection pH?Na⁺and K⁺and erythrocyte intracellular Ca²⁺,cell membrane PS before the operation in both groups.The pH?Na⁺and K⁺of IBS blood and the intracellular Ca²⁺and PS expression on membrane of IBS red blood cells were detected in the end of IBS in both groups.Results:1?pH?Na⁺?K⁺changes:?1?pH value:Compared the washing blood pH?7.723±0.098?with the arterial blood pH?7.381±0.021?of group N,the washing blood increased significantly?p<0.001?.Compared the arterial blood pH?7.402±0.029?with the washing blood?7.621±0.064?of group M,showed the washing blood a significant increase?p<0.001?.while the washing blood of group M?7.621±0.064?was lower than that of group N?7.723±0.098??p<0.05?.?2?Na⁺:Compared the arterial blood Na⁺?140±2?with the washing blood of group N Na⁺?150±3?,the washing blood increased significantly?p<0.001?.Compared the arterial blood Na⁺?140±3?with the washing blood of group M Na⁺?133±2?,the washing blood was significantly lower than that of the arterial blood?p<0.001?.Compared with the washing blood group N Na⁺?150±3?,group M Na⁺?133±2?decreased significantly?p<0.001?.?3?K⁺:compared the washing blood K⁺?0±0?with the arterial blood K⁺?3.9±0.4?of group N,the washing blood decreased significantly?p<0.001?.compared the washed blood K⁺?4.6±0.3?with the arterial blood k⁺?3.7±0.2?of group M,the washing blood increased significantly?p<0.001?,Compared group M washing blood K⁺?4.6±0.3?with the group N washed blood K⁺?0±0?,group M washed blood increased significantly?p<0.001?.2?The change of Ca²⁺positive of red blood cells:Compared the arterial blood Ca²⁺?1.79±0.26?%with the washing blood Ca²⁺?4.05±0.78?%of group N,the washing blood Ca²⁺?4.05±0.78?%increased significantly.?p<0.001?,compared the arterial blood Ca²⁺?1.57±0.31?%with the washing blood Ca²⁺?3.48±0.58?%of the group M,the washing blood Ca²⁺?3.48±0.58?%increased significantly?p<0.001?.compared the group M washing blood Ca²⁺?3.48±0.58?%with the group N washing blood Ca²⁺?4.05±0.78?%,the difference was not statistically significant?p>0.05?.3?The change of PS positive of cells membrane:Compared the arterial blood PS?0.57±0.28?%with the washing blood PS?2.30±0.36?%of group N,the washing blood PS?2.30±0.36?%of group N increased significantly?p<0.001?.Compared the arterial blood PS?0.47±0.26?%with the washing blood PS?0.82±0.24?%of group M,the washing blood PS?0.82±0.24?%of group M showed a significant increase?p<0.001?,Compared the group M washing blood PS?0.82±0.24?%with the group N washing blood PS?2.30±0.36?%the group M washing blood PS?0.82±0.24?%decreased significantly?p<0.001?.Conclusion:1?During the IBS process,the pH of the salvaged blood was higher than?7.35-7.45?the normal value after washed with normal saline or multiple electrolytes injection,but the multiple electrolytes injection was closer to the normal range.2?After washing with normal saline,Na⁺was higher than?135-145mmol/L?the normal range,and K⁺content was extremely low.while Na⁺was slightly lower than normal and K⁺was in?3.5-5.5mmol/L?the normal range after washing with multiple electrolytes injection.3?The Ca²⁺and PS positive in IBS red blood cells after washing with normal saline and multiple electrolytes injection was higher than that of the arterial blood.there was no difference in the positive of Ca²⁺between the two groups of washed blood,However,multiple electrolytes injection could reduce the positive of PS expression.