Experimental Study Of Artemisinin On The Renal Protective Effect In Diabetic Nephropathy Rats

Objective:To investigate the effect of artemisinin on renal protection and Rho A/ROCK signaling pathway in patients with spontaneous diabetic nephropathy.Materials and methods:Modeling method with a triple,unilateral kidney removed high-fat + + STZ injection of small dose in order to establish the model of early diabetic nephropathy rats were treated for 12 weeks,respectively using biochemical and electron microscopy and immunohistochemistry method in rats of vital signs,renal function,renal pathology and ultrastructure and immune Flt-1 immunohistochemical expression analysis.Immunohistochemistry paraffin sections of renal tissues of rats in the TGF-beta 1,Rho A,ROCKl,alpha-SMA and E-Cad protein expression.Using in situ hybridization on paraffin sections of renal tissues of rats in the TGFbeta 1,Rho A,ROCKl,-SMA and E-Cad alpha m RNA expression levels were detected.All the experimental data were analyzed using SPSS 19 statistical software.Result:The model of early diabetic nephropathy can be successfully replicated by the 1.triad modeling method.Artemisinin can not only improve the survival state,blood sugar,blood lipid and hemorheology indexes,but also improve the renal function,renal pathology and ultrastructure of rats,and also can down regulate the expression of Flt-1 in renal tissue.Renal tubular epithelial cells in 2.normal control group there was a small amount of TGF-1,Rho A,ROCKl and beta alpha-SMA protein,the expression level was significantly lower than the model group(P < 0.05);compared with the model group,in addition to artemisinin in small dose group,the treatment group of renal tubular epithelial cells in TGF-,Rho A,ROCKl beta 1 alpha and-SMA protein expression were decreased(P < 0.05);each group of artemisinin dose and high dose group decreased most significantly,the two groups had no significant difference(P > 0.05).Compared with the model group,normal control group,renal tubular epithelial cell cytoplasm E-Cadherin protein expression more(P < 0.05);compared with the model control group,in addition to artemisinin in small dose group,increased expression of E-Cadherin protein in treatment group,the rest of the cytoplasm of the renal tubular epithelial cells(P <0.05),middle dose group of artemisinin in each group and the high dose group decreased most significantly,the two groups had no significant difference(P > 0.05).Pressure3.normal control group of renal tubular epithelial cells in the presence of a small amount of TGF-1,Rho A,ROCKl and beta alpha-SMAm RNA expression,its expression level lower than the model control group(P < 0.05);compared with the model group,in addition to artemisinin in small dose group,the treatment group of renal tubular epithelial cells in TGF-,Rho A,ROCK1 1 beta and alpha the expression of-SMAm RNA was lower(P < 0.05),and high dose groups of artemisinin were decreased most significantly.Compared with the model control group,normal control group,m RNA E-Cadherin expression of renal tubular epithelial cell cytoplasm,high expression level((P < 0.05);compared with the model group,in addition to artemisinin in small dose group,the treatment group of renal tubular epithelial cell cytoplasm E-Cadherin expression of m RNA were higher(P < 0.05),the most.Obviously,in the large dose group of renal tubular epithelial cell cytoplasm E-Cadherinm RNA expression of artemisinin in each group,there was significant difference(P < 0.05).PressureConclusion:1.Artemisinin can be quantified by reducing the 24-hour urine protein of diabetic nephropathy rats,and can reduce the thickening of the glomerular basement membrane and the broadening of the mesangial matrix,thus providing a protective effect for the kidney.2.Artemisinin can improve the blood glucose,blood lipid and hemorheology of diabetic nephrotic rats,thus protecting the diabetic nephropathy.3.Artemisinin is able to cut the kidney tissues to decide TGF-1,Rho A,ROCK1,alpha SMA protein and m RNA expression,increase E-Cadherin protein and m RNA expression,thus speculated that artemisinin can inhibit renal interstitial fibrosis,delay the development of diabetic nephropathy.