Unsanitary Environment Reduce The Effect Of Antibiotics On Intestinal Microecology In Adult Mice

A number of studies have shown that the hygienic hypothesis is closely related to the occurrence of autoimmune diseases such as eczema,allergies,asthma,sclerosis,and autism.Our previous studies found that sanitation of living environment affected the diversity and structure of the intestinal microbiota in mice.It is reported that the diversity and species of intestinal microbiota affected the development of the body's immune and nervous system,so it is possible that the incidence of autoimmune and neurological diseases are related to the environmental sanitation by gut microbiota.Antibiotics are an important influencing factor in the intestinal microecology and are commonly used in clinical practice.It has not been reported whether the effect of antibiotics on the intestinal microflora is same under different sanitation of living environment.In this experiment,mice were used as research objects,and high-throughput sequencing technology was used to analyze the effects of antibiotics on the intestinal microecology of mice under different sanitation of environment.It was compared which factor of diet and environment are more dominant in reducing intestinal microecological disorders caused by antibiotics.Experimental content: We control the gene,mode of delivery,breeding mice and other conditions of the experimental subjects to ensure that factors are consistent.The mice were divided into five groups: One group was kept in the SPF animal house(fed normal diet);two groups were housed in the SPF animal room and leaked fecal(one group was fed a high-fat diet and other group was fed a normal diet);two groups were kept in general animal room(one group was fed a high-fat diet and other group was fed a normal diet).Mice were treated with antibiotics at 13 weeks.Before the antibiotic treatment(antibiotic treated immediately after the sample was collected),on the day of antibiotic withdrawal,on the third and the ninth day the feces were collected respectively.Then,the total bacterial DNA in feces was extracted,the 16 S r RNA gene of bacterial was Sequenced by Mi Seq high-throughput sequencer to analyze the diversity and structure of gut microbiota.Experimental results:(1)Unsanitary environment reduced the impact of antibiotics on the diversity and richness of gut microbiota in mice.(2)Unsanitary environment reduced the impact of antibiotics on the structure of gut microbes in mice(3)Unsanitary environment speeded up the recovery of intestinal microbiota that was treated by antibiotic.(4)Compared with Environmental sanitation,diets have no significant effect in reducing antibiotic-induced intestinal microbial disturbances.(5)Leaking fecal exacerbated the effect of antibiotics on the structure of intestinal microflora in mice.Conclusion: Unsanitary environment can reduce the effect of antibiotics on intestinal microecology in mice.