NAC1/HMGB1 Signaling Pathway Is Associated With Epithelial Mesenchymal Transition,Invasion And Metastasis Of Lung Cancer Cell Line

Objective:This study aims to investigate the role of NAC1/HMGB1 signaling pathway in the epithelial mesenchymal transition,invasion and metastasis of lung cancer cell line.Methods:Human lung cancer cell line A549 was used in this study.They were randomly divided into normal control group,sh-NAC1 empty vector group(sh-NAC1 NC),expression empty vector group(NAC1 NC),NAC1-shRNA and NAC1 over-expression group(NAC1).NAC1 and HMGB1 expression levels were detected by qRT-PCR method.Cell proliferation was detected by CCK8 kit.Cell cycles were detected by flow cytometry method.Cell invasion was detected by Transwell method.The expression levels of E-cadherin,N-cadherin,NAC1 and HMGB1 were detected by qRT-PCR and Western blotting mehods.Results:Compared with the control group,the expression level of NAC1 and cell proliferation in NAC1-shRNA group decreased significantly,cells in G1 phase increased significantly and cells in S phasedecreased significantly.In NAC1-shRNA group,E-cadherin expression levels increased significantly and the expression levels of N-cadherin,HMGB1 and Vimentin decreased significantly.In NAC1 group,the expression level of NAC1 and cell proliferation increased significantly,cells in S phase increased significantly and cells in G1 phase decreased significantly,E-cadherin expression levels decreased significantly and the expression levels of N-cadherin,HMGB1 and Vimentin increased significantly.Conclusions:The expression of NAC1 and HMGB1 in lung cancer cells may affect the occurrence of EMT,the NAC1/HMGB1 signaling pathway is associated with the EMT,invasion and metastasis of lung cancer cell.