Effects Of Liujunzi Decoction-containing Serum On The Expression Of E-cadherin And N-cadherin In A549/DDP Cells

Purpose:To study the effect of serum containing Liu Junzi decoction oncisplatin resistance remission of human adenocarcinoma of lung cell line A549/DDP,and the expression of E-cadherin and N-cadherin in A549/DDP.Material and method:The serum containing Liu Junzi decoction was prepared by serum pharmacology method,40 SD rats were divided into serum control group and serum containing Liu Junzi decoction(high,middle and low dose)groups.Liu Junzi decoction was orally given once a day for five days,serum was obtained.The serum was diluted into 5% and 10% with nutrient solution,and was added into A549/DDP for 12h?24h?48h.The cells were divided into 4 groups: A549 cell+blank group,A549/DDP cell+blank group,A549/DDP cell+ TGF-?1 induced group,and A549/DDP cell+TGF-?1 induced drug-containing group,the proliferation of A549/DDP cells was observed by MTT assay,and the the reversal fold was computed.The expressions of E-cadherin and N-cadherin proteins were examined by immunohistochemistry and western blot.Results: 1.Liu Junzi Decoction inhibited the growth and proliferation of 549/DDP cells obviously,and with a concentration-dependent and time-dependent manner,10% serum containing middle dose Liu Junzi Decoction cultured for 48 h has better effect and with no toxin.2.TGF-?1 induced IC50 of A549/DDP cells decreased(P<0.05),the reverse rate was 4.12.3.The serum containing Liu Junzi decoction increased the expression level of E-cadherin and reduced the expression level of N-cadherin.Conclusion: 1.The serum containing Liu Junzi decoction moderately reversed the multidrug resistance of A549/DDP cells,which probably is related to inhibiting epithelial mesenchymal transformation and increasing the sensitivity of 549/DDP to DDP.