Study On Extraction, Separation, Puriifcation And The Hypoglycemic Activity Of Morus Nigra Linn In Xinjiang

Objective: Study on the optimum extraction process of polysaccharides in Morusnigra Linn leaves of Xinjiang. Separation and purification of polysaccharides from Morusnigra Linn leaves of Xinjiang. Study on nature, monosaccharide or polysaccharidecomposition and hypoglycemic activity of polysaccharides from Morus nigra Linn leavesof Xinjiang. Methods:1. Using the orthogonal test method to determine leaves of Morusnigra Linn polysaccharide optimum extraction process.2. Using DEAE-52, SephadexG-200on leaves of Morus nigra Linn polysaccharides which was separated and purifiedunder the optimum extraction process. Determination the relative molecular mass.3.Study on the physicochemical properties of the isolated and purified polysaccharidefractions. Monosaccharide composition were analyzed by TLC. The obtainedpolysaccharide compounds were analyzed using UV, IR.4. Using GC to further determinethe composition of polysaccharides.5. Establishment of alloxan induced diabetic micemodel. Observation of Morus nigra Linn leaves polysaccharides the effect of bloodglucose and liver glycogen on diabetic mice. Analysis of its possible mechanism. Results:1. Morus nigra Linn leaves polysaccharides of Xinjiang optimum extraction process: Theextraction temperature is100℃, extraction time is3h,80%alcohol precipitationconcentration, solid-liquid ratio1:20, extracting for2times, material to liquid pH=7.0.2.Using the optimal extraction process of extraction of crude polysaccharide. Comparisonthe Sevage method and the TCA method of removing protein, Sevage method for removalof protein is good. After DEAE-52separated, crude polysaccharide get6polysaccharidefractions of W1, W2, N1, N2, N3and N4. Purification the4fractions of W1, N1, N2andN4by Sephadex G-200, the elution peaks are single symmetrical peaks. Determination ofthe polysaccharide molecular weight from21374to71331.3. Polysaccharides are notcontaining nucleic acid and protein and preliminary judgment of its componentmonosaccharides as alpha pyranose.4. Through the GC analysis W1of rhamnose, glucose,xylose, Arabia mannose, glucose, galactose six monosaccharides composition, molarcomposition ratio of7.16:9.59:1.09:1.05:12.36:15.52; N1consists of rhamnose, Arabia sugar, glucose and galactose four monosaccharides composition, mole ratio of5.25:5.76:9.28:11.66; N2and N4mainly composed of glucose and galactose twomonosaccharides composition, mole ratio of3:4.5. Morus nigra Linn leavespolysaccharides can reduce the level of blood glucose concentration, remission of alloxantype mouse pancreatic islet beta cell injury, promote glycogen synthesis function.Conclusion:1. Through the verification experiment shows that the polysaccharide yieldresults and the results of orthogonal experiment approach. Description of the process isfeasible.2. Separation and purification of Morus nigra Linn leaves polysaccharides, peakelution are single symmetrical peaks, preliminary judgment can be obtained component ispure. Molecular weight determination to lay the foundation for further structure analysisof Polysaccharides.3. Ultraviolet scanning the polysaccharide component does notcontain protein nucleic acid, that is completely removing protein. TLC methodpreliminary judgement of Morus nigra Linn leaves polysaccharides four component ofmonosaccharide composition. IR initially identified containing characteristic functionalgroup of Morus nigra Linn leaves polysaccharides.4. The important premise of studyingpolysaccharide structure is clear molar composition of monosaccharides inPolysaccharides. Through the GC analysis get4polysaccharides molar proportion.5.Preliminary may account for Morus nigra Linn leaves polysaccharides of Xinjiang canreduce blood sugar values.