Study On The Role Of HCG And LH/HCG Receptors In Regulating Endometrial Receptivity

Objective: To study the effects of low-dose(150 IU)HCG in the early follicular phase of frozen-thawed embryo transfer(FET)on clinical outcomes at 7 days after embryo transfer and the high-dose(10000 IU)HCG on endometrial receptivity of FET triggers.The role of LH/HCG receptor overexpression in the regulation of endometrial receptivity.Methods: The first study,FET patients were divided into experimental group and control group according to whether the early follicular phase was intramuscularly injected with small dose(150 IU)of HCG to 7 days after embryo transfer,including 184 cases in the experimental group and 104 cases in the control group.General information,pregnancy status,endometrial morphology and blood flow were statistically analyzed.In the second study,the FET patients were divided into natural cycle experimental group,natural cycle control group,artificial cycle experimental group and artificial cycle control group according to the periodic protocol whether or not the daily intramuscular injection of high dose(10000 IU)HCG.Endometrial tissue proteins were extracted and integrin ?3,leukemia inhibitory factor,and complement C3 factor were detected by Western Blot.The three endometrial cell cultures of normal population were extracted.The primary endometrial cells were transfected with LHCGR overexpressing lentivirus.The transfection rate was observed to be about 80% under fluorescence microscope.The cell protein was extracted and detected by Western blot.Integrin ?3,leukemia inhibitory factor,complement C3 factor.Result:(1)FET early follicular phase continuous intramuscular injection of small dose(150 IU)HCG to 7 days after embryo transfer,compared with the control group,the endometrial thickness thickening on the transplant day,the clinical pregnancy rate increased,the most subperitoneal blood flow The number of branches increased,and the differences were statistically significant(P<0.05).(2)There was no statistically significant expression of integrin ?3,leukemia inhibitory factor and complement C3 factor between the FT triggers and the high-dose(10000 IU)HCG natural cycle test group,the natural cycle control group,the artificial cycle test group and the artificial cycle control group.difference.(3)LHCGR overexpresses lentiviruses and transfects primary cells of endometrium.AtMOI=150,P infection reagents have the highest transfection efficiency.Compared with the Lentivirus group,the expression of integrin ?3,LIF and complement C3 was higher in the lentivirus group than in the Negative group,and the difference was statistically significant(P<0.05).Conclusion:(1)(1)FET patients with early intravesical injection of small doses of HCG to 7 days after embryo transfer,can improve the clinical pregnancy rate by improving endometrial thickness and blood flow.The FET patient's trigger intramuscular injection of large doses of HCG showed no significant improvement in endometrial receptivity.(2)Overexpression of LHCGR in isolated endometrial cells,which can up-regulate the endometrial receptivity factor.