| Background:The development and research of biomedical materials is becoming more and more important in the repair of organ tissue engineering.As a kind of biomedical material,the implant material is mainly used to replace the damaged and missing human hard tissue and to make it function in parallel.Titanium and titanium alloys have been widely used in implant materials due to their excellent mechanical properties and biocompatibility,but because they have no ability to induce new bone formation,that is,they have no biological activity and no antibacterial properties,so clinically,it is often necessary to modify the surface of the titanium material to have a certain performance for therapeutic purposes.The inflammatory response at the implant site is one of the clinically common postoperative complications,mainly due to bacterial infection.The preparation of coatings with antibacterial properties is an effective method to prevent bacterial infections,and the coatings also need to have good biocompatibility to ensure good bonding of the implant material to the bone.Objective:1.To determine the effect of different concentrations of lanthanum on the performance of titanium dioxide coating,and provide a theoretical basis for further guiding the surface topography of implants.2.Exploring the proper concentration of the ruthenium to make the coating have a long-lasting antibacterial ability on the basis of good biological activity.3.In view of the crucial role of osteoblasts in osseointegration,this study focuses on the interaction of osteogenic precursor cells with the surface of the material,providing a basis for more rational implant design.Methods:Titanium dioxide coatings with different concentrations of cerium were prep ared by micro-arc oxidation method by changing the composition of the electr olyte.Scanning electron microscopy(SEM),X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS)were used.Atomic force microscopy(AFM)and other related characterization of the surface morphology and structure of t he coating.The bioactivity of the material was evaluated by culturing osteoge nic precursor cells,gingival fibroblasts,on the surface of the material.The a ntibacterial properties of the materials were tested by culturing E.coli and St aphylococcus aureus on the surface of the material.Results:The cerium-containing titanium dioxide coating was successfully prepared on the surface of Ti-6Al-4V.The incorporation of cerium did not change the surface morphology and phase composition of the coating.The contact angle was also found to be hydrophilic.It did not change because of the change in the concentration of germanium contained in the coating.The cross-section EDS can be found to be uniformly distributed in the coating.Cellular experiments showed that osteogenic precursor cells and fibroblasts exhibited better cell proliferation ability on the microarc oxidation coating containing strontium.The expression of ALP,TGF-? and BMP-2 increased on the surface of the osteogenic precursor cells from day 1,3,and 7 of the culture.The amount of alizarin red stained calcium nodules was also incorporated with the concentration of strontium.The amount increases and increases.Bacterial experiments have found that low concentrations of antimony-containing coatings have no significant antibacterial properties against bacteria,but high concentrations of antimony-containing coatings have certain antibacterial properties.Conclusion:This study reveals for the first time the biocompatibility and antibacterial ability of titanium dioxide-coated coatings,and has developed an ideal new orthopedic and dental implant restoration material for the selection of materials for bone tissue repair and reconstruction. |
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