Expression Analysis Of FGFR3 In Different Periods Of Rat Denervated Callus

Objective:FGFR3 is a member of the fibroblast growth factor receptor family and belongs to the tyrosine kinase family and plays an important role in skeletal development.If the expression is poor,it can cause a variety of systemic diseases.Moreover,the specificity of this factor receptor is that it is the only one in its family that negatively regulates bone growth.After nerve injury,the factor receptor is highly expressed,mainly in cartilage osteogenesis.For the pathological osteophytes produced after nerve injury,there is a sufficient macroscopic understanding in the clinic.This experiment aims to explore the role of this factor receptor in the process of fracture healing by making a rat model of denervated tibia fracture,using the negative regulation of this factor receptor and injecting FGFR3 inhibitor.The influence of structure and the law of expression change.It is hoped that through this research,some adverse effects caused by denervation will be improved,and the understanding of pathological osteophytes under denervation will be deepened,which will provide reference for further research and provide theoretical guidance for understanding and treating such fractures in the future.Methods : Sixty female SD rats were randomly divided into two groups,experimental group and control group.The experimental group made a humeral transverse fracture model of the sacral nerve injury,and intraperitoneal injection of FGFR3 blocker.The control group was identical to the experimental group,and the same dose of normal saline was given after operation.They were sacrificed at 4,7,10,14 and 21 days after surgery,and then X-ray films were taken(each group = 6 at each time point).The bone density was first scanned,then fixed and decalcified,and the fracture line was taken.A section of the tibia 4 mm above and below was embedded in paraffin,cut into 5um thick sections,and each specimen was stained with HE,Masson three-color staining,and histopathological analysis.Graphical analysis software was used to collect data on the growth of osteophytes in pathological photographs,and statistical analysis of the experimental data was performed.Results : Compared with the control group,the fracture healing of the experimental group was accelerated,the number of trabecular bone was more,the density of the epiphysis around the fracture was significantly increased,and the reconstruction and shaping of the epiphysis were accelerated(P<0.05).The expression changes of FGFR3 showed a certain regularity.The data of each group showed that the receptors were most active during the 7-14 days after fracture.Conclusion:FGFR3 significantly inhibits the growth and maturation of callus during the healing of denervated fractures.Inhibition of FGFR3 can accelerate fracture healing and promote the shaping of the epiphysis in the case of peripheral nerve denervation.At the same time,the expression of this factor receptor is time-specific,and its expression peak period is 7-14 days after fracture.