Expression And Clinical Significance Of Plasma MiR-424, MiR-92a And MiR-130b In Esophageal Squamous Cell Carcinoma

Objective:To investigate the relationship between the expression levels of miR-424,miR-92a and miR-130b in patients with esophageal squamous cell carcinoma?ESCC?and healthy people,and to explore the relationship between clinicopathological features and esophageal squamous cell carcinoma,to evaluate its diagnostic value in esophageal squamous cell carcinoma.Methods:1.The expression levels of plasma microRNA-424,microRNA-92a and microRNA-130b in patients with esophageal squamous cell carcinoma and healthy people.The Department of Thoracic and Cardiovascular Surgery of the First Affiliated Hospital of Chengdu Medical College,collected from 70 patients with esophageal squamous cell carcinoma from January 2017 to May 2018,and 60 plasma samples from healthy people.The plasma total RNA was isolated and extracted for purity determination and analysis.The qualified samples were reverse transcribed,cDNA was synthesized,and the miRNA primer sequences of the target were obtained and synthesized.Quantitative real-time polymerase chain reaction?RT-PCR?was used to detect esophageal squamous cell carcinoma patients and healthy people.The expression levels of miR-424,miR-92a and miR-130b in plasma were different,and 2^-??Ct was the relative expression of target gene,2^-?Ct was the expression level of esophageal squamous cell carcinoma or healthy people,??Ct esophageal squamous cell carcinoma=Ct esophageal squamous cell carcinoma-Ct internal reference,?Ct healthy people=Ct healthy people-Ct internal reference,??Ct=?Ct esophageal squamous cell carcinoma-?Ct healthy people?.2.Relationship between plasma miR-424,miR-92a and miR-130b and clinicopathological features of esophageal squamous cell carcinoma.RT-PCR was used to detect the expression of miR-424,miR-92a and miR-130b in plasma of patients with esophageal squamous cell carcinoma at different stages,and to explore the relationship between expression level and clinicopathological features of esophageal squamous cell carcinoma.3.Diagnostic value of plasma miR-424,miR-92a and miR-130b in esophageal squamous cell carcinoma.The receiver operating characteristic curve?ROC?was used to calculate the specificity,sensitivity and area under the curve?AUC?of plasma miR-424,miR-92a and miR-130b for diagnosis of esophageal squamous cell carcinoma,to evaluate the diagnostic value of the above three plasma miRNAs in esophageal squamous cell carcinoma.Results:1.The expression levels of miR-424,miR-92a and miR-130b in plasma of patients with esophageal squamous cell carcinoma were significantly up-regulated compared with healthy people,and the difference was statistically significant?P<0.001?;2.There was no significant difference in plasma miR-424 expression level and esophageal squamous cell carcinoma grade,differentiation,lymph node metastasis and tumor stage?P>0.05?.Plasma miR-92a expression levels are significantly higher in patients with esophageal squamous cell carcinoma with lymph node metastasis than in patients without lymph node metastasis?P=0.001?;and the expression of plasma miR-92a in patients with TNM?esophageal squamous cell carcinoma was significantly higher than that in patients with TNM I and II?P=0.001;P=0.007?.Plasma miR-130b expression was significantly higher in patients with esophageal squamous cell carcinoma with lymph node metastasis than in patients without lymph node metastasis?P=0.025?;at the same time,plasma levels of miR-130b in patients with TNM?esophageal squamous cell carcinoma were significantly higher than those in patients with TNM I and II?P<0.001?.The expression levels of plasma miR-424,miR-92a and miR-130b in age,sex and tumor location of patients with esophageal squamous cell carcinoma were not statistically significant.3.The corresponding AUC for plasma miR-424,miR-92a and miR-130b for the diagnosis of esophageal squamous cell carcinoma were 0.759?95%CI:0.674 to 0.844?,0.749?95%CI:0.668 to 0.836?and 0.833?95%CI:0.759 to 0.906?;specificity and sensitivity were 83.33%and 62.86%,71.67%and 74.29%,86.67%and 77.14%,respectively.4.The AUC of miR-424 and miR-92a,miR-424 and miR-130b,miR-92a and miR-130b for the diagnosis of esophageal squamous cell carcinoma were 0.813?95%CI:0.735-0.876??0.853?95%CI:0.780-0.909?and 0.849?95%CI:0.775-0.906?,specificity and sensitivity were 83.33%and 68.57%,76.67%and 85.71%,78.33%and82.86%,respectively.The aortic diagnosis of esophageal squamous cell carcinoma with miR-424,miR-92a and miR-130b was 0.873?95%CI:0.804-0.925?,and the sensitivity and specificity were 87.14%and 76.67%,respectively.Conclusion:1.The expression levels of plasma miR-424,miR-92a and miR-130b in patients with esophageal squamous cell carcinoma were significantly higher than those in healthy people;2.The expression levels of plasma miR-92a and miR-130b increased with lymph node metastasis and TNM staging in patients with esophageal squamous cell carcinoma,suggesting that these two miRNAs may play an important role in esophageal squamous cell carcinoma;3.Compared with single miRNA,plasma miRNA combination can improve the diagnostic value of esophageal squamous cell carcinoma.The combination of miR-424?miR-92a and miR-130b is more suitable as a biological marker for the diagnosis of esophageal squamous cell carcinoma.