Phytochemical Investigations Of Atraphaxis Spinosa And Camphorosma Lessingii Litv.

Phytochemical investigation of the methanol extract from the entire plant of Atraphaxis spinosa L from Armenia led to the isolation of quercitrin(1),afzelin(2),N-trans-p-coumaroyldopamine(3),N-trans-feruloyldopamine(4),quercetin 3-O-?-D-glucuronide-6''-methyl ester(5),myricitrin(6),quercetin-3-O-?-D-glucuronide(7),2”-galloyl-quercetin-3-O-?-D-glucuronide(8),2-(?-D-glucopyranosyloxy)-4-hydroxy-6-methoxyacetophenone(9),fisetinidol(10),methyl gallate(11),gallic acid(12),?-linolenic acid(13),loliolide(14),N-trans-feruloyltyramine(15),and lucidulactone A(16).The structures were elucidated by 1D and 2D homonuclear and heteronuclear NMR spectroscopy and by comparison with closely related compounds published previously.In the present research,compounds 1,2,5,7-9,11,13,14,16 are reported for the first time from genus Atraphaxis.Compounds 5,8,9,14,16 have not been reported previously from any species in the Polygonaceae.The chemotaxonomic significance of the isolated compounds is discussed.Phytochemical investigation of Camphorosma lessingii Litv.has resulted in the isolation of four previously unreported isoflavones(1-4),and eight known compounds(5-12).Nine of these compounds(1-6,8-10)are reported for the first time from members of the family Amaranthaceae.The structures of all isolated compounds were determined by spectroscopic methods,primarily 1D and 2D NMR and mass spectrometry.The absolute configuration of 6 was confirmed by circular dichroism.Inhibition of the organic anion transporters,OAT1 and OAT3,by the isolated compounds was evaluated.Among them,7,2'-dihydroxy-6,8-dimethoxyisoflavone(1),2'-hydroxy-6,7,8-trimethoxyisoflavone(2),6,2'-dihydroxy-7,8-dimethoxyisoflavone(3),7-methoxyflavone(5)showed a significant inhibitory effect on the 6-CF uptake mediated by OAT1 and OAT3.