Studies On The Chemical Constituents Of N-Butanol Parts Of Tinosporae Sinenisis

Kuang-jin-teng is the dried vine stem of Tinosporae sinenisis(Lour.)Merr.(Menispermaceae).Its is bitter in taste and neutral in cold,acts on the liver m eridian,and has effect of relief swelling and pain,for the bruises sprains,rheuma tism.We study on the chemical constituents of n-butanol extract from Tinospor ae sinenisis(Lour.)Merr.Using silica gel,ODS,Sephadex LH-20 column chrom atography and combining preparative HPLC,21 compounds were isolated and e lucidated on the basis of spectroscopic and chemical methods,namely Tinosine nside A(1)?4-Imidazolidinone-2-(isopropyl)-5-(phenylmethyl)(2)?1-[2-(furan-2-yl)-2-oxoethyl]pyrrolidin-2-one(3)?Syringin(4)?corchoionoside C(5)?Cordysi nin B(6)?3,4-dimethoxyphenyl-?-D-glucopyranoside(7)?Cinnamol-O-?-D-gluco pyranoside(8),3,4,5-trimethoxylphenol-?-D-glucopyranoside(9)?Benzyl-?-D-gl ucopyranoside(10)?syringic acid(11)?3,5-Dimethoxybenzoic acid-4-O-?-D-gluco pyranoside(12)?1,3-Benzenediethanol,4,6-dimethoxy(13)? Lyciumide A(14)?Tyroosine(15)? [cyclo-(-Leu-Ile)](16)?(-)-episyringaresino(17)?(+)-syringares inol-4?-O-?-D-glucopyranoside(18)?(+)-syringaresinol(19)?(+)-lyoniresin-4-yl ?-D-glucopyranoside(20)?(-)-8?-epilyoniresin-4-yl ?-glucopyranoSide(21).Compo unds 1 and 2 is new,and compounds 3?5?6?13?14?15?16 are isolated fr om Tinosporae for thefirst time.In addition,experiment of two new compounds against A?25-35 induced cytotoxicity,showed,showed protective effects at a certain concentration.