Expression Of FOXO3 In Clinical Samples Of Patients With Gastric Cancer And Its Effect On The Malignant Behavior Of Human Gastric Adenocarcinoma AGS Cells

Objective: Previous studies have shown that transcription factor FOXO3 in cells can bind to various target genes in the nucleus and participate in regulating various cell biological processes such as cell proliferation,apoptosis and autophagy,but currently there are few studies on the expression and mechanism of FOXO3 in gastric cancer and gastric cancer cells using relevant databases.The aim of our study is to investigate the roles of FOXO3 in gastric cancer and its relevant mechanism.Metabolic gEne RApid Visualizer database was used to analyze the clinical data of FOXO3 in gastric cancer and futher validate the expression of FOXO3 in our own clinical samples.In addition,we investigate the effect of overexpression of FOXO3 on the proliferation,migration and invasion of human gastric adenocarcinoma AGS cell line in vitro.Methods: Metabolic gEne RApid Visualizer database was used to detect the transcriptional level of transcription factor FOXO3 in GC.Clinical samples of 15 pair patients with GC were collected and its corresponding normal normal tissues.The real-time fluorescent quantitative PCR was used to determine the transcription level of FOXO3 in a small sample of clinical specimens.The CCK8 assay was used to detect the proliferation of human gastric adenocarcinoma AGS cells in vitro.The effect of FOXO3 on the migration and invasion of human gastric adenocarcinoma AGS cells was evaluated by wound healing assay and Transwell assay.The gastric adenocarcinoma AGS cell line was treated with PI3 K inhibitor LY294002,and the expression of PI3K/Akt pathway-related protein was detected by Western blot.Results: FOXO3 showed low transcription level in most GC tissue samples analyzed by Metabolic gEne RApid Visualizer database.The results of our own clinical samples showed that the expression level of FOXO3 mRNA in gastric cancer tissues is significantly lower than that in adjacent tissues.CCK8,wound healing assay and Transwell showed that the effect of transcription factor FOXO3 on the proliferation,migration and invasion ability in vitro of AGS cells were obviously inhibited.The results of Western blot showed that FOXO3 appears to be a downstream effector of PI3K/Akt pathway.Conclusions: According to the analysis of large sample data of Metabolic gEne RApid Visualizer database combined with the validation of our own clinical samples,the expression of FOXO3 in gastric cancer patients is low compared with the normal group,and also inhibits the proliferation,migration and invasion of AGS cells.This laid the foundation of suppressor effect with FOXO3 in gastric cancer in the future.Furthermore,the results showed that anticancer therapies targeting FOXO3 may be useful in the treatment of gastric cancer.