Comparison Of Different Muscle-Relaxant Anesthetics On Growth,Migration And Invasion Of Gastric Cancer Cells

Objective:The skeletal muscular relaxants is called muscle relaxants,also known as neuromuscular blocking agents,can be affiliated with N₂ receptor and block nerve impulses to the muscles and are always used in surgery for general anesthesia.Gastric cancer is currently one of the most common malignant tumors in the world,and it is the leading cause of cancer-related death diseases worldwide.China is one of the high-risk areas of gastric cancer around world.At present,radical resection is still the most ideal treatment for gastric cancer,but the main reason of death for those people are recurrence and/or metastasis.Recently,increasing numbers of reports suggested that anesthetic techniques and anesthetic drugsmay affect the activity of tumor cells,including growth,invasion and migration.However,the effect of muscle-relaxant anestheticson cell activity in gastric cancer is currently unknown.The present study aimed to examine and compare the role of three different muscle-relaxant anesthetics including Rocuronium bromide?Rb?,Vecuronium bromide?Vb?and Cisatracurium Besilate?CB?in gastric cancer cells.Methods:Gastric cancer cells?SGC7901 and BGC 823?were inoculated in culture plates,cultured to logarithmic growth phase and treated with a different dose of muscle-relaxants anesthetics,Rocuronium bromide?8?g/ml,80?g/ml?,Vecuronium bromide?1.5?g/ml,15?g/ml?and Cisatracurium Besilate?2.5?g/ml,25?g/ml?.Control groups were not exposed to the Rb,Vb,and CB.Using in vitro models,the cancer cells were subjected to several experiments,such as cell proliferation assay with CCK-8,scratch wounding assay,adhesion assay and migration assay.Results:Cell proliferation assay with CCK-8 results show that,compared with the untreated control group,Rb resulted in a significant increase in gastric cancer cell growth,in SGC7901?p<0.05?and BGC823 cells?p<0.05?,when treated with regular blood concentration and a tenfold blood concentration,though a concentration dependent effect was not observed.When SGC7901and BGC823 cells were cultured with Vb.High concentration Vb marginally promoted cell proliferation,whilst low concentration had no effect.CB did not result in a significant change on the growth of gastric cancer cells,but the tenfold could result in partial cell death.The scratch wounding assayresults showed that Rb facilitated the movement of SGC7901 and BGC823 cells compared with the untreated control both at 8?g/ml and80?g/ml and reached statistical significance?p<0.05?.This increase in SGC7901 gastric cancer cell migration was also observed when treated with Vb,however this did not reach statistical significance.This trend was not observed in BGC823 gastric cancer cells.High CB concentration could also increase cell migration.The adhesionassay results showed that both in SGC7901 and BGC823 cell lines,Rb and Vb promoted cell adhesion in a concentration dependent manner.CB had no significant effect on the adhesive properties of gastric cancer cells.Invasion assay results showed that Rb significantly increased the invasive potential of SGC7901 and BGC823 cells but CB did not.Invasion did not change upon treatment with Vb at a regular blood concentration,however a tenfold blood concentration facilitated cell invasion.Conclusion:We found that Rb increased the growth,invasion and migration of gastric cancer cells SGC7901 and BGC823.However,Vb and CB,did not significantly affect gastric cancer cells'malignant phenotype at their regular blood concentration.Our results are important in selecting type and dose of anesthetic used for surgery of gastric cancer patients.However further molecular and clinical studies are required to unravel the mechanisms or pathways of muscle relaxants on cancer cells.