Jiao-tai-wan Attenuates Intestinal Inflammation And Permeability Through Inhibiting TLR4/MyD88/NF-?B Signaling Pathway In Insomnic Rats

Purpose: The aim of this study was to investigate the effects of long-term partial sleep deprivation on the glucose metabolism and intestinal epithelial barrier function in obesity-resistant rats,and to explore the mechanism of Jiao-tai-wan in attenuating systemic inflammation and glucose metabolism disorders.Methods: Male obesity-resistant SD rats were sleep deprived for 16 weeks.During the last 8 weeks,they were treated with Jiao-tai-wan.Intestinal permeability was detected with the FITC-dextran perfusion assay method,plasma LPS levels were detected with Tachypleus Amebocyte Lysate.The concentration of serum insulin,proinflammation cytokines IL-1?,IL-6,TNF-? and plasma LBP were determined by ELISA.The m RNA levels of IL-1?,IL-6,TNF-? of intestine were detected by PCR.Western bolt was used in signaling pathway analysis.Results: Sleep deprivation could activate the intestinal TLR4/NF-?B pathway and increase the expression of IL-6 and TNF-?.This effect increased the intestinal permeability and the circulation LPS,LBP,IL-6 and TNF-?.The ratio of p-Akt/Akt of epididymal white adipose tissue was decreased.Treatment of Jiao-tai-wan could partly reversed these changes.Conclusion: Jiao-tai-wan had a potential to attenuate disturbance of glucose metabolism,peripheral inflammation and insulin resistant in partially sleep deprived rats.These effects may relate to suppressing the local inflammation of intestine induced by insomnia and repairing the tight junction to decrease the gut permeability.