| Objective: In intraoperative radiotherapy for pancreatic cancer,healthy pancreatic tissue is appropriated to a fair dose.It uses a single high-dose electron beam to irradiate the pancreatic region of male SD rats to simulate the radiation injury of rat pancreas under intraoperative radiotherapy.I see the general condition,blood glucose,body weight,diet and pancreatic histopathology of SD rats theoretical character data for pancreatic injury caused by intraoperative radiotherapy for pancreatic cancer.Method: It divided forty male Sprague-Dawley rats aged 10-12 weeks old with no specific pathogen(SPF)into the experimental group and control group: 8 in the control group and 32 in the experimental group.Applying the practical patent of the instructor,the “small animal radiation accurate irradiation test bench”,a single 8MeV 20 Gy irradiated the pancreatic region of the experimental group.Results: After a single 20 Gy 8MeV electron beam emission,the rats in the preliminary group showed the preliminary group showed a decrease in train and indifference in the first 2 weeks.The launch started better in the fourth week,the management of scouring and drinking escalated,and the spiritual state developed.The week was related to the management the state group fiber loss occurred at the first week;it shed the fourth week clean;the skin was bare,and new grass grew out to was at the 8th week.The dietary dose decreased reduced in the and 8th days(P<0.05),and built up after the 12 th day,and was presented to simple(P>0.05).The carcass weight decreased reduced in the week,and the group weight raised in the second week(P<0.05).The weight gain was the fastest in the 4th to 6th week,and there was no difference in the weight between the two groups at the 3rd week and later(P>0.05).The rats in the control group were normal,smooth;the diet and body weight had been stable.The experimental group showed a decrease in blood glucose at the second week after irradiation,which differed from the control group(p<0.05).There was no significant difference in blood glucose between the two groups at week 4(p=0.79).There was a significant difference between the 6th week and the 8th week(p<0.05).The dose of SD rats fluctuated after irradiation.Pearson correlation analysis between tasting peripheral blood glucose and dietary dose showed that the two indicators were irrelevant,ρ=0.12,p>0.05.Pathological view: In the experimental group,the pancreas was swollen,grayish white,and adhered to surrounding tissues,and the boundary was unclear.The pancreas of the control group was grayish pink and cleared from the surrounding adipose tissue.HE stains examination: under the light microscope,the pancreatic cells of pancreatic exocrine showed pancreatic interstitial and islet cell edema in the pancreatic cells at the second week,accompanied by inflammatory cell infiltration.In the 4th week,the individual gland cells were necrotic and atrophic,and it disordered islet cells;at the 6th week,it reduced the glandular cells of the pancreatic atrophy,and something started the fibrosis around the interstitial and islet cells;in the 8th week,the pancreatic water disappeared,and the atrophic gland cells were almost invisible,but the interstitial fibrosis was obvious.There were fibrous tissues around the islet cells.There were no abnormal changes in pancreatic gland cells and islet cells in the experimental group.Conclusion: 1.A single 8MeV electron line 20 Gy irradiated the pancreatic area of SD rats to cause abnormal pancreatic function.Although peripheral blood glucose fluctuations occurred,the blood glucose levels were normal in the 2nd,4th,6th,and 8th week after irradiation,and inflammation occurred in the early stage after normal pancreas radiation.2.Cell infiltration formed fibrous repair over time,and no serious complications such as the large fake necrosis of pancreatic material developed.3.It shows it to tolerate this dose in healthy pancreatic tissue.But whether it can tolerate higher doses is for further study. |
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